Abstract |
A simple and reliable method was established for simultaneous determination of 4-hydroxyphenyl acetic acid, 4-hydroxyphenyl lactic acid, and 3,4-hydroxyphenyl propionic acid in human urine by high-performance liquid chromatography with fluorescence detection. Solid-phase extraction was used to eliminate the interferences in urine. The separation of three analytes was achieved using a C18 column and a mobile phase formed by a 95:5 v/v mixture of 50 mmol/L ammonium acetate buffer at pH 6.8 that contained 5 mmol/L tetrabutyl ammonium bromide and acetonitrile. Under the optimized conditions, the detection limits of 4-hydroxyphenyl acetic acid, 4-hydroxyphenyl lactic acid, and 3,4-hydroxyphenyl propionic acid were 4.8 × 10-3 , 8.80 × 10-3 , and 9.00 × 10-3 mg/L, respectively, and the recoveries were in the range of 85.0-120.0% with relative standard deviations of 1.5-3.1%. This method was used to analyze urine samples from breast cancer patients, healthy people and post-surgery breast cancer patients. Significant differences in urinary levels of 4-hydroxyphenyl acetic acid and 4-hydroxyphenyl lactic acid could be found between the breast cancer patients group and other two groups. No effect of age and sex was observed on the urinary levels of 4-hydroxyphenyl acetic acid and 4-hydroxyphenyl lactic acid. This method might be helpful for cancer biomarkers discovery in urine.
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Authors | Yongli Yang, Fan Liu, Yiqun Wan |
Journal | Journal of separation science
(J Sep Sci)
Vol. 40
Issue 10
Pg. 2117-2122
(May 2017)
ISSN: 1615-9314 [Electronic] Germany |
PMID | 28346746
(Publication Type: Journal Article)
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Copyright | © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
- Acetates
- Propionates
- Lactic Acid
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Topics |
- Acetates
(urine)
- Breast Neoplasms
(urine)
- Chromatography, High Pressure Liquid
- Female
- Humans
- Lactic Acid
(urine)
- Limit of Detection
- Male
- Propionates
(urine)
- Solid Phase Extraction
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