[Western Blot diagnostic yield for simultaneous antibody-detection in patients with human cysticercosis, hydatidosis, and human fascioliasis].

Abstract

OBJECTIVES:

. To determine the diagnostic yield using western blotting to simultaneously detect antibodies in patients with human cysticercosis, hydatidosis, and human fascioliasis. Materials and methods . Cross-sectional study of diagnostic yield assessment. Excretory/secretory antigens were obtained from Taenia solium larvae, Echinococcus granulosus cysts, and the adult flukes of Fasciola hepática, which were then separated using the polyacrylamide gel electrophoresis technique, transferred, and attached to a nitrocellulose membrane to be probed with sera from the patient infected with the three parasites. The sensitivity of the technique was assessed using 300 individual serum samples, 60 pools of two parasites, and 20 pools of three parasites with 75 sera from patients with other parasites, 10 from patients with other diseases, and 15 from patients without parasites. Results . The technique revealed 13 glycoproteins (GP): GP 35, 31, 24, 23, 18, 17, 14, and 13 kDa for cysticercosis; GP 8, 16, and 21 kDa for hydatidosis; and GP 17 and 23 kDa for fascioliasis. The test detected the presence of antibodies with a sensitivity of 96% (95% confidence interval [CI] = 94.62-98.54%) in the detection of one or the thirteen bands, a specificity of 100% (95% CI = 99.50-100.00%); individually, there was a sensitivity for cysticercosis of 97% (95% CI = 93.16-100.00%), for hydatidosis of 94% (95% CI = 88.85-99.15%) and for fascioliasis of 96% (95% CI = 91.66-100.00%). Conclusions . Western blotting is effective in the simultaneous detection of antibodies in patients with human cysticercosis, hydatidosis, and fascioliasis, and it can be used as a diagnostic test to either rule out or confirm the presence of antibodies in endemic areas.

• Authors: Kelly Davelois, Hermes Escalante, César Jara
• Journal: Revista peruana de medicina experimental y salud publica (Rev Peru Med Exp Salud Publica) 2016 Oct-Dec Vol. 33 Issue 4 Pg. 616-624 ISSN: 1726-4642 [Electronic] Peru
• Vernacular Title: Rendimiento diagnóstico del Western Blot para detectar simultaneamente anticuerpos en pacientes con cisticercosis, hidatidosis y fascioliasis humana.
• PMID: 28327829 (Publication Type: Journal Article)

Chemical References

• Antibodies, Helminth
• Antigens, Helminth

Topics

• Animals
• Antibodies, Helminth (analysis)
• Antigens, Helminth
• Blotting, Western
• Cross-Sectional Studies
• Cysticercosis (diagnosis)
• Echinococcosis (diagnosis)
• Enzyme-Linked Immunosorbent Assay
• Fascioliasis (diagnosis)
• Humans
• Sensitivity and Specificity