An imaging target TGF-β1 for hepatocellular carcinoma in mice.

Abstract

It has been reported that the positive detection rate of Fluorine-18-fluorodeoxyglucose (18F-FDG) metabolism in positron emission tomography (PET) imaging, for the diagnosis of hepatocellular carcinoma (HCC) is only about 50%. In particular, 18F-FDG PET imaging is prone to false negative findings in HCC. Transforming growth factor-beta1 (TGF-β1) shows over expression rates in early HCC liver tissue growth and promotes tumor invasion and metastases. Our aim was to use In this study, we used the feasibility of iodine-125 (125I)-labeled TGF-β1 antibody as a nuclear medicine imaging target in HCC.

MATERIALS AND METHODS:

The TGF-β1 antibody was obtained from Bioss Inc. The Huh-7 cell line (Liver Cancer Institute, Zhongshan Hospital, Fudan University) is a HCC cell line with high metastatic potential. Each mouse was subcutaneously injected with 5×106/0.1mL Huh-7 cells in the right upper flank region for the establishment of a subcutaneous xenograft model. The Iodogen method was used to label TGF-β1 antibody with 125I. In this experiment, 100μL of 125I- TGF-β1 antibody solution, which contained approximately 18,5MBq of 125I-liraglutide, was injected into the tail veins of each of three nude mice with Huh-7 HCC. Micro SPET/CT imaging was performed for each mouse using a nano SPET/CT.

RESULTS:

The average percentage of injected dose per gram of tissue (ID%) was 1,3% and 2,4%. The tumor was strongly positive for TGF-β1.

CONCLUSION:

This pilot study provides an experimental basis for further exploration of the feasibility of TGF-β1 receptor as a target in HCC imaging and in other cancers.

• Authors: Yiqiu Zhang, Beilei Li, Xiao Li, Hui Tan, Dengfeng Cheng, Hongcheng Shi
• Journal: Hellenic journal of nuclear medicine (Hell J Nucl Med) 2017 Jan-Apr Vol. 20 Issue 1 Pg. 76-78 ISSN: 1790-5427 [Print] Greece
• PMID: 28315912 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Biomarkers, Tumor
• Iodine Radioisotopes
• Radiopharmaceuticals
• Transforming Growth Factor beta1

Topics

• Animals
• Antibodies, Monoclonal (pharmacokinetics)
• Biomarkers, Tumor (metabolism)
• Carcinoma, Hepatocellular (diagnostic imaging, metabolism)
• Cell Line, Tumor
• Iodine Radioisotopes (pharmacokinetics)
• Liver Neoplasms (diagnostic imaging, metabolism)
• Male
• Metabolic Clearance Rate
• Mice
• Mice, Inbred C57BL
• Mice, Nude
• Molecular Diagnostic Techniques (methods)
• Radiopharmaceuticals (pharmacokinetics)
• Reproducibility of Results
• Sensitivity and Specificity
• Single Photon Emission Computed Tomography Computed Tomography (methods)
• Tissue Distribution
• Transforming Growth Factor beta1 (metabolism)