Abstract |
Rats injected with by d- phenylalanine, a carbonic anhydrase (CA) activator, enhanced spatial learning, whereas rats given acetazolamide, a CA inhibitor, exhibited impairments of fear memory consolidation. However, the related mechanisms are unclear. We investigated if CAs are involved in a non-spatial recognition memory task assessed using the object recognition test (ORT). Systemic administration of acetazolamide to male CD1 mice caused amnesia in the ORT and reduced CA activity in brain homogenates, while treatment with d- phenylalanine enhanced memory and increased CA activity. We provided also the first evidence that d- phenylalanine administration rapidly activated extracellular signal-regulated kinase (ERK) pathways, a critical step for memory formation, in the cortex and the hippocampus, two brain areas involved in memory processing. Effects elicited by d- phenylalanine were completely blunted by co-administration of acetazolamide, but not of 1-N-(4-sulfamoylphenyl-ethyl)-2,4,6-trimethylpyridinium perchlorate (C18), a CA inhibitor that, differently from acetazolamide, does not cross the blood brain barrier. Our results strongly suggest that brain but not peripheral CAs activation potentiates memory as a result of ERK pathway enhanced activation.
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Authors | Lucas Canto de Souza, Gustavo Provensi, Daniela Vullo, Fabrizio Carta, Andrea Scozzafava, Alessia Costa, Scheila Daiane Schmidt, Maria Beatrice Passani, Claudiu T Supuran, Patrizio Blandina |
Journal | Neuropharmacology
(Neuropharmacology)
Vol. 118
Pg. 148-156
(05 15 2017)
ISSN: 1873-7064 [Electronic] England |
PMID | 28286213
(Publication Type: Journal Article)
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Copyright | Copyright © 2017 Elsevier Ltd. All rights reserved. |
Chemical References |
- 1-N-(4-sulfamoylphenylethyl)-2,4,6-trimethylpyridinium
- Carbonic Anhydrase Inhibitors
- Pyridinium Compounds
- Sulfonamides
- Phenylalanine
- Extracellular Signal-Regulated MAP Kinases
- Carbonic Anhydrases
- Acetazolamide
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Topics |
- Acetazolamide
(pharmacology)
- Analysis of Variance
- Animals
- Carbonic Anhydrase Inhibitors
(pharmacology)
- Carbonic Anhydrases
(metabolism)
- Cerebral Cortex
(drug effects, enzymology)
- Dose-Response Relationship, Drug
- Drug Administration Routes
- Extracellular Signal-Regulated MAP Kinases
(metabolism)
- Hippocampus
(drug effects, enzymology)
- Male
- Mice
- Motor Activity
(drug effects)
- Phenylalanine
(pharmacology)
- Phosphorylation
(drug effects)
- Pyridinium Compounds
(pharmacology)
- Recognition, Psychology
(drug effects, physiology)
- Sulfonamides
(pharmacology)
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