Abstract | INTRODUCTION: To clarify the difference between system A and L amino acid transport imaging in PET clinical imaging, we focused on the use of α-[N-methyl-11C]-methylaminoisobutyric acid ([11C] MeAIB), and compared it with [S-methyl-11C]- L-methionine ([11C]MET). The aim of this study was to assess the correlation of accumulation of these two radioactive amino acid analogs with expression of amino acid transporters and cell proliferative activity in carcinoma cells. METHODS: RESULTS AND CONCLUSION: [14C] MeAIB uptake occurred principally via a Na+-dependent A type mechanism whereas [3H]MET uptake occurred predominantly via a Na+-independent L type mechanism although other transporters were also utilized depending on cell type. There was no correlation between [3H]MET uptake and total system L amino acid transporter (LAT) expression. In contrast, [14C] MeAIB uptake strongly correlated with total system A amino acid transporter (SNAT) expression and proliferative activity in this preliminary study using four human carcinoma cell lines. Carcinoma proliferative activity also correlated with total SNAT expression. Advances in Knowledge and Implications for Patient Care: Because there is a significant correlation between the accumulation of [14C] MeAIB and the gene expression level of total SNAT as well as the accumulation of [3H]FLT, it is suggested that use of the analog [11C] MeAIB in PET may provide an indication of tumor cell proliferative activity. [11C] MeAIB is therefore expected to be very useful in PET imaging.
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Authors | Shinya Kagawa, Ryuichi Nishii, Tatsuya Higashi, Hiroshi Yamauchi, Emi Ogawa, Hiroyuki Okudaira, Masato Kobayashi, Mitsuyoshi Yoshimoto, Naoto Shikano, Keiichi Kawai |
Journal | Nuclear medicine and biology
(Nucl Med Biol)
Vol. 49
Pg. 8-15
(Jun 2017)
ISSN: 1872-9614 [Electronic] United States |
PMID | 28284101
(Publication Type: Comparative Study, Journal Article)
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Copyright | Copyright © 2017 Elsevier Inc. All rights reserved. |
Chemical References |
- Amino Acid Transport Systems
- beta-Alanine
- 2,2-dimethyl-beta-alanine
- Methionine
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Topics |
- Amino Acid Transport Systems
(genetics)
- Biological Transport
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Gene Expression Regulation, Neoplastic
(drug effects)
- Humans
- Methionine
(metabolism)
- Pilot Projects
- Positron-Emission Tomography
- beta-Alanine
(analogs & derivatives, metabolism, pharmacology)
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