Abstract | AIM: To clarify the mechanisms involved in the critical endoplasmic reticulum (ER) stress initiating unfolded protein response pathway modified by melatonin. METHODS:
Hepatoma cells, HepG2, were cultured in vitro. Flow cytometry and TUNEL assay were used to measure HepG2 cell apoptosis. Western blotting and quantitative reverse transcription-polymerase chain reaction methods were used to determine the protein and messenger RNA levels of ER stress and apoptosis related genes' expression, respectively. Tissue microarray construction from patients was verified by immunohistochemical analysis. RESULTS: CONCLUSION: These findings indicate that melatonin as a novel selective ATF-6 inhibitor can sensitize human hepatoma cells to ER stress inducing apoptosis.
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Authors | Li-Jia Bu, Han-Qing Yu, Lu-Lu Fan, Xiao-Qiu Li, Fang Wang, Jia-Tao Liu, Fei Zhong, Cong-Jun Zhang, Wei Wei, Hua Wang, Guo-Ping Sun |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 23
Issue 6
Pg. 986-998
(Feb 14 2017)
ISSN: 2219-2840 [Electronic] United States |
PMID | 28246472
(Publication Type: Journal Article)
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Chemical References |
- ATF6 protein, human
- Activating Transcription Factor 6
- Antioxidants
- BAX protein, human
- BCL2 protein, human
- CCAAT-Enhancer-Binding Proteins
- Proto-Oncogene Proteins c-bcl-2
- RNA, Messenger
- RNA, Small Interfering
- bcl-2-Associated X Protein
- Tunicamycin
- CEBPE protein, human
- Cyclooxygenase 2
- PTGS2 protein, human
- Melatonin
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Topics |
- Activating Transcription Factor 6
(antagonists & inhibitors, genetics)
- Antioxidants
(pharmacology)
- Apoptosis
(drug effects)
- CCAAT-Enhancer-Binding Proteins
(metabolism)
- Cyclooxygenase 2
(metabolism)
- Down-Regulation
- Endoplasmic Reticulum Stress
(drug effects)
- Hep G2 Cells
- Humans
- In Situ Nick-End Labeling
- Liver Neoplasms
- Melatonin
(pharmacology)
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- RNA Interference
- RNA, Messenger
(metabolism)
- RNA, Small Interfering
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
- Tunicamycin
(pharmacology)
- Unfolded Protein Response
(drug effects)
- bcl-2-Associated X Protein
(metabolism)
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