Abstract | BACKGROUND: METHODS: Here we investigated the anti- cancer effects and mechanisms of ZJW, CC, ER, berberine, and evodiamine in cells and in intrahepatic xenograft mice. RESULTS: Treatment of HepG2 cells with ZJW, CC, ER, berberine, and evodiamine significantly displayed cytotoxic effects in a dose- and time-dependent manner. Hierarchical cluster analysis of gene expression profiles showed that CC and ZJW shared a similar mechanism for the cytotoxic effects, suggesting that CC was the active ingredient of ZJW for anti- cancer activity. Network analysis further showed that c-myc was the likely key molecule involved in the regulation of ZJW-affected gene expression. A human hepatoma xenograft model was established by intrahepatic injection of HepG2 cells containing nuclear factor-κB-driven luciferase genes in immunocompetent mice. In vivo bioluminescence imaging showed that cells had been successfully transplanted in mouse liver. Oral administration of ZJW for 28 consecutive days led to a significant decrease in the accumulation of ascites, the ratio of tumor-to-liver, and the number of transplanted cells in livers. CONCLUSIONS: In conclusion, our findings suggested for the first time that ZJW significantly suppressed human cancer cell growth in orthotopic HepG2 xenograft-bearing immunocompetent mice. Moreover, c-myc might play a potent role in the cytotoxic mechanisms of ZJW, CC, ER, berberine, and evodiamine.
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Authors | Shun-Ting Chou, Chien-Yun Hsiang, Hsin-Yi Lo, Hui-Fen Huang, Ming-Tsung Lai, Ching-Liang Hsieh, Su-Yin Chiang, Tin-Yun Ho |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 17
Issue 1
Pg. 121
(Feb 20 2017)
ISSN: 1472-6882 [Electronic] England |
PMID | 28219365
(Publication Type: Journal Article)
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Chemical References |
- Alkaloids
- Antineoplastic Agents, Phytogenic
- Drugs, Chinese Herbal
- Quinazolines
- Zuo-Jin-Wan
- Berberine
- evodiamine
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Topics |
- Alkaloids
(pharmacology, therapeutic use)
- Animals
- Antineoplastic Agents, Phytogenic
(pharmacology, therapeutic use)
- Berberine
(pharmacology, therapeutic use)
- Carcinoma, Hepatocellular
(drug therapy)
- Coptis
(chemistry)
- Drugs, Chinese Herbal
(chemistry, pharmacology, therapeutic use)
- Evodia
(chemistry)
- Female
- Hep G2 Cells
- Heterografts
- Humans
- Medicine, Chinese Traditional
- Mice, Inbred ICR
- Phytotherapy
- Quinazolines
(pharmacology, therapeutic use)
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