Abstract |
The survival kinase Akt has clinical relevance to radioresistance. However, its contributions to the DNA damage response, DNA double strand break ( DSB) repair and apoptosis remain poorly defined and often contradictory. We used a genetic approach to explore the consequences of genetic alterations of Akt1 for the cellular radiation response. While two activation-associated mutants with prominent nuclear access, the phospho-mimicking Akt1-TDSD and the clinically relevant PH-domain mutation Akt1-E17K, accelerated DSB repair and improved survival of irradiated Tramp-C1 murine prostate cancer cells and Akt1-knockout murine embryonic fibroblasts in vitro, the classical constitutively active membrane-targeted myrAkt1 mutant had the opposite effects. Interestingly, DNA- PKcs directly phosphorylated Akt1 at S473 in an in vitro kinase assay but not vice-versa. Pharmacological inhibition of DNA- PKcs or Akt restored radiosensitivity in tumour cells expressing Akt1-E17K or Akt1-TDSD. In conclusion, Akt1-mediated radioresistance depends on its activation state and nuclear localization and is accessible to pharmacologic inhibition.
|
Authors | S Oeck, K Al-Refae, H Riffkin, G Wiel, R Handrick, D Klein, G Iliakis, V Jendrossek |
Journal | Scientific reports
(Sci Rep)
Vol. 7
Pg. 42700
(02 17 2017)
ISSN: 2045-2322 [Electronic] England |
PMID | 28209968
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- 8-dibenzothiophen-4-yl-2-morpholin-4-yl-chromen-4-one
- Chromones
- DNA-Binding Proteins
- Heterocyclic Compounds, 3-Ring
- MK 2206
- Morpholines
- Nuclear Proteins
- Piperazines
- Protein Kinase Inhibitors
- Pyrimidines
- ipatasertib
- DNA
- AKT1 protein, human
- DNA-Activated Protein Kinase
- Prkdc protein, mouse
- Proto-Oncogene Proteins c-akt
|
Topics |
- Animals
- Apoptosis
(drug effects)
- Cell Line, Tumor
- Chromones
(pharmacology)
- DNA
(genetics, metabolism)
- DNA Breaks, Double-Stranded
- DNA End-Joining Repair
- DNA-Activated Protein Kinase
(antagonists & inhibitors, genetics, metabolism)
- DNA-Binding Proteins
(antagonists & inhibitors, genetics, metabolism)
- Epithelial Cells
(drug effects, metabolism, pathology)
- Fibroblasts
(cytology, drug effects, metabolism)
- Heterocyclic Compounds, 3-Ring
(pharmacology)
- Humans
- Male
- Mice
- Morpholines
(pharmacology)
- Mutation
- Nuclear Proteins
(antagonists & inhibitors, genetics, metabolism)
- Phosphorylation
(drug effects)
- Piperazines
(pharmacology)
- Prostate
(drug effects, metabolism, pathology)
- Protein Kinase Inhibitors
(pharmacology)
- Proto-Oncogene Proteins c-akt
(genetics, metabolism)
- Pyrimidines
(pharmacology)
- Radiation Tolerance
(drug effects)
- T-Lymphocytes, Regulatory
(drug effects, metabolism, pathology)
|