Anti-thrombotic and pro-fibrinolytic effects of levosimendan in human endothelial cells in vitro.

Abstract

AIMS:

Levosimendan is an inodilator for the treatment of acute decompensated heart failure (HF). Data from clinical studies suggest that levosimendan is particularly effective in HF due to myocardial infarction. After acute revascularization, no reflow-phenomenon is a common complication that may lead to pump failure and cardiogenic shock. Our aim was to examine whether levosimendan interferes with the pro-thrombotic phenotype of activated endothelial cells in vitro.

METHODS:

Human heart microvascular endothelial cells (HHMEC) and human umbilical vein endothelial cells (HUVEC) were treated with interleukin-1β (IL-1β) (200U/mL) or thrombin (5U/mL) and co-treated with or without levosimendan (0.1-10μM) for 2-24h. In addition, flow experiments were performed. Effects on plasminogen activator inhibitor-1 (PAI-1) and tissue factor (TF) expression and activity were measured by rt-PCR, specific ELISA and flow cytometry.

RESULTS:

Treatment with IL-1β or thrombin significantly increased the expression of PAI-1 and TF in endothelial cells. Co-treatment with levosimendan strongly attenuated the effects of IL-1β and thrombin on PAI-1 and TF mRNA by up to 50% and 45%, in a dose- and time-dependent manner. Similar results were obtained under flow conditions. Furthermore, co-treatment with levosimendan dampened the antigen production of PAI-1 and the surface expression of TF by 35% and 45%, respectively. Additionally, levosimendan diminished both TF and PAI-1 activity.

CONCLUSION:

Levosimendan down-regulates the expression of the pro-thrombotic and anti-fibrinolytic biomolecules TF and PAI-1 in activated human endothelial cells. Our findings may, at least in part, explain some of the beneficial effects of levosimendan after myocardial reperfusion.

• Authors: Konstantin A Krychtiuk, Christoph Kaun, Philipp J Hohensinner, Stefan Stojkovic, Jacqueline Seigner, Stefan P Kastl, Andreas Zuckermann, Wolfgang Eppel, Sabine Rauscher, Rainer de Martin, Gerald Maurer, Kurt Huber, Johann Wojta, Walter S Speidl
• Journal: Vascular pharmacology (Vascul Pharmacol) Vol. 90 Pg. 44-50 (03 2017) ISSN: 1879-3649 [Electronic] United States
• PMID: 28192257 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier Inc. All rights reserved.

Chemical References

• Fibrinolytic Agents
• Hydrazones
• Interleukin-1beta
• Plasminogen Activator Inhibitor 1
• Pyridazines
• SERPINE1 protein, human
• Simendan
• Thromboplastin
• Thrombin

Topics

• Cells, Cultured
• Dose-Response Relationship, Drug
• Down-Regulation
• Fibrinolysis (drug effects)
• Fibrinolytic Agents (pharmacology)
• Human Umbilical Vein Endothelial Cells (drug effects, metabolism)
• Humans
• Hydrazones (pharmacology)
• Interleukin-1beta (pharmacology)
• Plasminogen Activator Inhibitor 1 (metabolism)
• Pyridazines (pharmacology)
• Simendan
• Thrombin (pharmacology)
• Thromboplastin (metabolism)
• Time Factors