Abstract | BACKGROUND: OBJECTIVE: METHODS: C6 astrocyte cell line was pre-incubated in the presence or absence of resveratrol (100 μM) for 1 hour. After pre-incubation, resveratrol was maintained and 5 mM ammonia was added for 24 hours, followed by the evaluation of ROS production, mitochondrial functionality, antioxidant enzymatic and non-enzymatic defenses, energy metabolic parameters, and genotoxicity. RESULTS: We showed that resveratrol prevented the increase in ROS production, the decrease of mitochondrial membrane potential (ΔΨm), and bioenergetics deficit caused by ammonia in C6 astroglial cells. In addition, resveratrol avoided the ammonia-induced upregulation of NOX activity and impairment in enzymatic and non-enzymatic antioxidant defenses. Ammonia also induced DNA damage that was prevented by resveratrol, indicating its genoprotective effect. CONCLUSIONS: In summary, our study demonstrates that resveratrol prevents ammonia-induced cytotoxicity, as well as supports the role of resveratrol on mitochondrial/cellular redox functionality.
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Authors | Larissa Daniele Bobermin, Diogo Onofre Souza, Carlos-Alberto Gonçalves, André Quincozes-Santos |
Journal | Nutritional neuroscience
(Nutr Neurosci)
Vol. 21
Issue 4
Pg. 276-285
(May 2018)
ISSN: 1476-8305 [Electronic] England |
PMID | 28165879
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Reactive Oxygen Species
- Stilbenes
- Ammonia
- Catalase
- Glutathione Peroxidase
- Creatine Kinase
- Glutathione
- Resveratrol
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Topics |
- Ammonia
(toxicity)
- Animals
- Antioxidants
(pharmacology)
- Astrocytes
(drug effects, metabolism)
- Catalase
(metabolism)
- Cell Line
- Creatine Kinase
(metabolism)
- DNA Damage
(drug effects)
- Glutathione
(metabolism)
- Glutathione Peroxidase
(metabolism)
- Membrane Potential, Mitochondrial
- Mitochondria
(drug effects, metabolism)
- Oxidation-Reduction
(drug effects)
- Oxidative Stress
(drug effects)
- Rats
- Reactive Oxygen Species
(metabolism)
- Resveratrol
- Stilbenes
(pharmacology)
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