The involvement of endoplasmic reticulum stress response in immune dysfunction of dendritic cells after severe thermal injury in mice.

Abstract

Suppressed adaptive immune function is one of the major concerns responsible for the development of opportunistic infections and subsequent sepsis with high mortality in severe burns. Endoplasmic reticulum stress (ERS) is the endogenous self-protective mechanism, and it plays an important role in almost every process of living by regulating the balance between homeostasis and apoptosis. The current study investigated the involvement of ERS in the pathogenesis of dysfunction of dendritic cells (DCs) in burn mice. Our results show a significant ERS response in splenic DC after burn injury. Treatment with salubrinal (Sal, reported to protect cells against ERS-induced apoptosis.) decrease the apoptotic rate of DC induced by burns, and promote maturation and activation of DC, as well as the ability to promote T cell proliferation and polarization towards Th1 immunity (all P<0.05). Gene silence of XBP-1 (key molecular in ERS response) results in the increased apoptosis and suppressed phenotypical maturation of splenic DC in burn mice. These results show that the excessive ERS is essential for immunosuppression during severe thermal injury. XBP-1 plays a pivotal role in DC functional immunomodulation in burn mice. Inhibition of apoptotic ERS response benefits mice from major burns.

• Authors: Xiao-Mei Zhu, Ning Dong, Yan-Bo Wang, Qing-Hong Zhang, Yan Yu, Yong-Ming Yao, Hua-Ping Liang
• Journal: Oncotarget (Oncotarget) Vol. 8 Issue 6 Pg. 9035-9052 (Feb 07 2017) ISSN: 1949-2553 [Electronic] United States
• PMID: 28118617 (Publication Type: Journal Article)

Chemical References

• Cinnamates
• Cytokines
• X-Box Binding Protein 1
• Xbp1 protein, mouse
• salubrinal
• Thiourea

Topics

• Adaptive Immunity (drug effects)
• Animals
• Apoptosis
• Burns (drug therapy, immunology, metabolism, pathology)
• Cells, Cultured
• Cinnamates (pharmacology)
• Coculture Techniques
• Cytokines (immunology, metabolism)
• Dendritic Cells (drug effects, immunology, metabolism, pathology)
• Disease Models, Animal
• Endoplasmic Reticulum Stress (drug effects)
• Lymphocyte Activation
• Male
• Mice, Inbred BALB C
• Phenotype
• RNA Interference
• Severity of Illness Index
• Spleen (drug effects, immunology, metabolism, pathology)
• Th1 Cells (immunology, metabolism)
• Thiourea (analogs & derivatives, pharmacology)
• Time Factors
• X-Box Binding Protein 1 (genetics, immunology, metabolism)