Alpha-2-macroglobulin and heparin cofactor II and the vulnerability of carotid atherosclerotic plaques: An iTRAQ-based analysis.

Abstract

Rupture of carotid atherosclerotic plaque may cause stroke, while few biomarker in clinic can evaluate carotid plaque vulnerability. In this study, we divided the recruited participants into no plaque, stable plaque, and vulnerable plaque group according to carotid ultrasonography, and screened the differentially expressed proteins in plasma of these participants using isobaric tags for relative and absolute quantitation labeling coupled with liquid chromatography-tandem mass spectrometry. 28 proteins were identified differentially expressed, among which alpha-2-macroglobulin (α2M) and heparin cofactor II (HCII) were found to be at hub position in the interactions of these proteins by STRING analysis and were selected for enzyme-linked immunosorbent assay measurement to assess their relevance with carotid plaques vulnerability and diagnostic efficiency. The plasma level of α2M was found positively correlated, while HCII level was negatively correlated with higher vulnerability of carotid plaques. Both proteins were efficient in differentiating stable and vulnerable carotid plaques. These findings provide potential new targets for the research of carotid plaque vulnerability. Plasma α2M and HCII may be potential biomarkers for evaluation of the vulnerability of carotid plaques if further studied.

• Authors: Jinmao Li, Xi Liu, Ying Xiang, Xueying Ding, Teng Wang, Ying Liu, Maojia Yin, Changhong Tan, Fen Deng, Lifen Chen
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 483 Issue 3 Pg. 964-971 (02 12 2017) ISSN: 1090-2104 [Electronic] United States
• PMID: 28087279 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2017 Elsevier Inc. All rights reserved.

Chemical References

• A2M protein, human
• Biomarkers
• SERPIND1 protein, human
• alpha-Macroglobulins
• Heparin Cofactor II

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers (blood)
• Blood Chemical Analysis (methods)
• Carotid Stenosis (blood)
• Female
• Heparin Cofactor II (metabolism)
• Humans
• Male
• Middle Aged
• Plaque, Atherosclerotic (blood)
• alpha-Macroglobulins (metabolism)