Effect of incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder on ACE inhibitory activity in fermented milk by L. plantarum LP69.

Abstract

BACKGROUND:

Angiotensin I converting enzyme (ACE) plays an important physiological role in regulating hypertension. Lactic acid bacteria are known to produce ACE inhibitory peptides which can lower hypertension during fermentation.

METHODS:

The effect of incubation time (0~36 h), inoculum size (3, 4, 5, 6 and 7%, v/v), temperature (25, 30, 35, 40 and 45°C), sterilization time (5, 10, 15, 20 and 25 min), concentration of goat milk powder (8, 10, 12, 14 and 16%, w/v) and whey powder (0.5, 0.6, 0.7, 0.8 and 0.9%, w/v) on ACE inhibitory peptides fermented from goat milk by Lactobacillus plantarum LP69 was investigated using single factor experiment.

RESULTS:

The optimal incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder in fermented milk by L. plantarum LP69 was 14 h, 3.0%, 35°C, 20 min, 14% and 0.70% for ACE inhibitory activity and 22 h, 3.0%, 40°C, 25 min, 16% and 0.60% for viable cell counts, respectively.

CONCLUSIONS:

The incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder had a significant influence on ACE inhibitory activity in fermented milk by Lactobacillus plantarum LP69, the results are beneficial for further screening of main factors by using fractional factorial designs.

• Authors: Guowei Shu, Hui Yang, He Chen, Qiuhong Zhang, Yue Tian
• Journal: Acta scientiarum polonorum. Technologia alimentaria (Acta Sci Pol Technol Aliment) 2015 Apr-Jun Vol. 14 Issue 2 Pg. 107-116 ISSN: 1898-9594 [Electronic] Poland
• PMID: 28068008 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Angiotensin-Converting Enzyme Inhibitors
• Antihypertensive Agents
• Bacterial Proteins
• Peptide Fragments
• Peptidyl-Dipeptidase A

Topics

• Angiotensin-Converting Enzyme Inhibitors (chemistry, metabolism, pharmacology)
• Animals
• Antihypertensive Agents (chemistry, metabolism, pharmacology)
• Bacterial Proteins (biosynthesis, chemistry, pharmacology)
• China
• Cultured Milk Products (analysis, microbiology)
• Food Handling
• Food, Preserved (analysis, microbiology)
• Goats
• Hot Temperature
• Lactobacillus plantarum (growth & development, metabolism)
• Microbial Viability
• Milk (chemistry, microbiology)
• Pasteurization
• Peptide Fragments (chemistry, metabolism, pharmacology)
• Peptidyl-Dipeptidase A (chemistry, metabolism)
• Protein Stability
• Rabbits
• Time Factors
• Whey (chemistry, microbiology)