Imaging, biodistribution and efficacy evaluation of 188Re-human serum albumin microspheres via intraarterial route in an orthotopic hepatoma model.

Abstract

PURPOSE:

Liver cancer is the second most common cause of death worldwide. This study was to investigate the SPECT/CT, ultrasound, biodistribution and therapeutic evaluation of 188Re-human serum albumin microspheres (188Re-HSAM) in the GP7TB orthotopic hepatoma rat model.

MATERIALS AND METHODS:

HSAM was labeled with 188Re by using a home-made kit and microwave system. The 188Re-HSAM was administered via intraarterial route. The in vivo distribution of 188Re-HSAM was determined by biodistribution analysis and nanoSPECT/CT system. In efficacy, tumor volumes were tracked longitudinally by three-dimensional ultrasound.

RESULTS:

The biodistribution and nanoSPECT/CT imaging showed that 188Re-HSAM could accumulate in liver and tumor. The correlation coefficient of tumor volumes done by three-dimensional ultrasound and at autopsy was 0.997. In efficacy, tumor volume in the normal saline-treated group was 1803.2 mm3 at 54 days after tumor inoculation. Tumor volumes in the 103.6 MBq and 240.5 MBq of 188Re-HSAM treated groups were 381 and 267.4 mm3 (p = 0.001 and 0.004), respectively.

CONCLUSIONS:

These results show that three-dimensional ultrasound with a high spatial resolution and contrast in soft tissue can become imaging modality in assessing tumor burden and tumor progression in an orthotopic rat model. The longitudinally therapeutic evaluation of 188Re-HSAM demonstrated dose-dependent tumor growth inhibition with increased dose in the GP7TB orthotopic hepatoma rat model.

• Authors: Liang-Cheng Chen, Ya-Jen Chang, Su-Jung Chen, Wan-Chi Lee, Chih-Hsien Chang, Te-Wei Lee, Jui-Hung Shien
• Journal: International journal of radiation biology (Int J Radiat Biol) Vol. 93 Issue 5 Pg. 477-486 (05 2017) ISSN: 1362-3095 [Electronic] England
• PMID: 28045339 (Publication Type: Journal Article)

Chemical References

• Capsules
• Radioisotopes
• Radiopharmaceuticals
• Serum Albumin
• Rhenium

Topics

• Animals
• Apoptosis (radiation effects)
• Capsules (chemical synthesis, pharmacokinetics)
• Carcinoma, Hepatocellular (diagnostic imaging, metabolism, radiotherapy)
• Cell Line, Tumor
• Injections, Intra-Arterial
• Liver Neoplasms (diagnostic imaging, metabolism, radiotherapy)
• Male
• Organ Specificity
• Radioisotopes (administration & dosage, pharmacokinetics)
• Radiopharmaceuticals (administration & dosage, pharmacokinetics)
• Rats, Inbred F344
• Rhenium (administration & dosage, pharmacokinetics)
• Serum Albumin (chemistry)
• Single Photon Emission Computed Tomography Computed Tomography
• Theranostic Nanomedicine
• Tissue Distribution
• Treatment Outcome
• Tumor Burden (radiation effects)
• Ultrasonography