Abstract | BACKGROUND: Although hepatic resection is a potentially curative treatment for hepatocellular carcinoma (HCC), post-operative prognosis remains unsatisfactory due to the high incidence of recurrence. Several clinicopathological markers have been associated with HCC recurrence, but none has been validated. Extratumoral expression of cytochrome P4501A2 ( CYP1A2) was recently proposed as predictor of HCC recurrence. AIMS: To validate extratumoral CYP1A2 as predictor of HCC recurrence and to determine its applicability to pretreatment liver biopsy. METHODS: Surgically resected HCC (n.180) with clinicopathological data and follow up were retrospectively studied (HCV n.54; HBV n.91; NAFLD/NASH n.35). CYP1A2 expression was evaluated using an immunohistochemical assay and semiquantitative analysis. RESULTS: Etiology-stratified analysis showed that low CYP1A2 expression was independently associated with recurrence-free survival in HCV patients (HR 2.814, 95% CI 1.300-6.093, p=0.009); this association was lost in the whole cohort. Pretreatment liver biopsy and paired surgical specimens showed concordant CYP1A2 expression in the vast majority of cases (87%), with NPV of 100%, PPV of 81.25%, and a Cohen kappa of 0.72 (substantial agreement). CONCLUSION: We validated the extratumoral expression of CYP1A2 as a biomarker of HCC recurrence in HCV patients. CYP1A2 analysis in pretreatment liver biopsy can be of help to stratify HCC patients for personalized treatment.
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Authors | Amedeo Sciarra, Bogdan Pintea, Ji Hae Nahm, Matteo Donadon, Emanuela Morenghi, Marco Maggioni, Jean Frederic Blanc, Guido Torzilli, Matthew Yeh, Paulette Bioulac-Sage, Young Nyun Park, Massimo Roncalli, Luca Di Tommaso |
Journal | Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
(Dig Liver Dis)
Vol. 49
Issue 4
Pg. 434-439
(Apr 2017)
ISSN: 1878-3562 [Electronic] Netherlands |
PMID | 28040498
(Publication Type: Journal Article, Multicenter Study, Validation Study)
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Copyright | Copyright © 2016 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved. |
Chemical References |
- CYP1A2 protein, human
- Cytochrome P-450 CYP1A2
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Topics |
- Aged
- Carcinoma, Hepatocellular
(pathology, surgery)
- Cytochrome P-450 CYP1A2
(metabolism)
- Female
- Hepatectomy
- Hepatitis C
(complications)
- Humans
- Incidence
- Italy
- Liver
(pathology)
- Liver Neoplasms
(pathology, surgery)
- Male
- Middle Aged
- Multivariate Analysis
- Neoplasm Recurrence, Local
(diagnosis, mortality)
- Prognosis
- Retrospective Studies
- Survival Analysis
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