Olfactory receptors are
G protein-coupled receptors. Some of them are expressed in
tumor cells, such as the OR51E2 receptor overexpressed in LNCaP
prostate cancer cells. It is considered a prostate
tumor marker. We previously demonstrated that this receptor is able to promote LNCaP cell invasiveness in vitro upon stimulation with its odorant agonist β-
ionone, leading to increased generation of
metastases in vivo. In the present study, we show that even a relatively short exposure to β-
ionone is sufficient to promote
metastasis emergence. Moreover, α-
ionone, considered an OR51E2 antagonist, in fact promotes prostate
tumor growth in vivo. The combination of α-
ionone with β-
ionone triggers a higher increase in the total
tumor burden than each molecule alone. To support the in vivo results, we demonstrate in vitro that α-
ionone is a real agonist of OR51E2, mainly sustaining LNCaP cell growth, while β-
ionone mainly promotes cell invasiveness. So, while structurally close, α-
ionone and β-
ionone appear to induce different cellular effects, both leading to increased
tumor aggressiveness. This behaviour could be explained by a different coupling to downstream effectors, as it has been reported for the so-called biased
ligands of other
G protein-coupled receptors.