Scutellarin suppresses migration and invasion of human hepatocellular carcinoma by inhibiting the STAT3/Girdin/Akt activity.

Abstract

Scutellarin is an active flavone from Erigeron breviscapine (vant) Hand Mass. This study aimed to investigate the potential role of scutellarin in migration and invasion of human hepatocellular carcinoma (HCC) cells and its possible mechanism. In comparison with the vehicle-treated controls, treatment with scutellarin (50 mg/kg/day) for 35 days significantly mitigated the lung and intrahepatic metastasis of HCC tumors in vivo. Scutellarin treatment significantly reduced HepG2 cell viability in a dose-dependent manner, and inhibited migration and invasion of HCC cells in vitro. Scutellarin treatment significantly reduced STAT3 and Girders of actin filaments (Girdin) expression, STAT3 and Akt phosphorylation in HCC cells. Introduction of STAT3 overexpression restored the scutellarin-downregulated Girdin expression, Akt activation, migration and invasion of HCC cells. Furthermore, induction of Girdin overexpression completely abrogated the inhibition of scutellarin on the Akt phosphorylation, migration and invasion of HCC cells. Scutellarin can inhibit HCC cell metastasis in vivo, and migration and invasion in vitro by down-regulating the STAT3/Girdin/Akt signaling.

• Authors: Yang Ke, Tianhao Bao, Xuesong Wu, Haoran Tang, Yan Wang, Jiayun Ge, Bimang Fu, Xu Meng, Li Chen, Cheng Zhang, Yuqi Tan, Haotian Chen, Zhitang Guo, Fan Ni, Xuefen Lei, Zhitian Shi, Dong Wei, Lin Wang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 483 Issue 1 Pg. 509-515 (01 29 2017) ISSN: 1090-2104 [Electronic] United States
• PMID: 27998773 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Antineoplastic Agents, Phytogenic
• CCDC88A protein, human
• Glucuronates
• Microfilament Proteins
• STAT3 Transcription Factor
• STAT3 protein, human
• Vesicular Transport Proteins
• scutellarin
• Apigenin
• Proto-Oncogene Proteins c-akt

Topics

• Animals
• Antineoplastic Agents, Phytogenic (pharmacology)
• Apigenin (pharmacology)
• Carcinoma, Hepatocellular (drug therapy, pathology)
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Glucuronates (pharmacology)
• Hep G2 Cells (drug effects, pathology)
• Humans
• Liver Neoplasms (drug therapy, pathology)
• Male
• Mice, Inbred BALB C
• Microfilament Proteins (antagonists & inhibitors, metabolism)
• Proto-Oncogene Proteins c-akt (antagonists & inhibitors, metabolism)
• STAT3 Transcription Factor (antagonists & inhibitors, metabolism)
• Vesicular Transport Proteins (antagonists & inhibitors, metabolism)
• Xenograft Model Antitumor Assays