USPIO enhanced lymph node MRI using 3D multi-echo GRE in a rabbit model.

Abstract

Ultrasmallsuperparamagnetic iron oxide (USPIO) has been suggested to be a negative MR contrast agent to detect metastatic lymph nodes. Previously reported studies have evaluated the diagnostic performance of USPIO-enhanced MR lymph node imaging based on signal intensity. In this study, we investigate the specific performance of three different parametric approaches (normalized signal intensity, R2 * and susceptibility) using 3D multi-echo gradient echo to quantify the USPIO particles in lymph nodes. Nine rabbits with VX2 tumor implants were scanned before and after USPIO injection. From 3D multi-echo GRE magnitude and phase data, we generated multi-echo combined T2 *-weighted images, an R2 * map, and a quantitative susceptibility map. Eighteen lymph nodes (nine reactive and nine metastatic) were evaluated and showed remarkable signal drops in the area of USPIO accumulation. On parametric analysis, the R2 * difference before and after USPIO injection was significantly different (p < 0.05) between reactive and metastatic lymph nodes; in contrast, the normalized signal intensity and susceptibility were not significantly different between the nodes. Our study showed the potential utility of USPIO-enhanced MRI using R2* mapping from 3D multi-echo GRE for the detection of lymph node metastasis and parametric analysis of lymph node status in a rabbit model. Copyright © 2016 John Wiley & Sons, Ltd.

• Authors: Sung Hun Kim, Soon Nam Oh, Hyun Seok Choi, Hyun Sil Lee, Jaeseop Jun, Yoonho Nam, Sung Hak Lee, Jin-Kwon Lee, Hae Giu Lee
• Journal: Contrast media & molecular imaging (Contrast Media Mol Imaging) Vol. 11 Issue 6 Pg. 544-549 (11 2016) ISSN: 1555-4317 [Electronic] England
• PMID: 27976506 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 John Wiley & Sons, Ltd.

Chemical References

• Dextrans
• Magnetite Nanoparticles
• ferumoxtran-10

Topics

• Animals
• Cell Line, Tumor
• Dextrans (pharmacology)
• Imaging, Three-Dimensional (methods)
• Lymph Nodes (diagnostic imaging)
• Lymphatic Metastasis (diagnostic imaging)
• Magnetic Resonance Imaging (methods)
• Magnetite Nanoparticles
• Rabbits