Inhibition of Tobacco Ringspot Virus by the Culture Fluid of L-Lysine-α-Oxidase Producing Strain.

Abstract

A producing strain of an anti-tumor and antiviral enzyme L-lysine-α-oxidase from Trichoderma was cultured using a technological device of G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (Pushchino). L-lysine-α-oxidase activity in the obtained metabolite concentrate was 5.4 U/ml. We studied the effects of the concentrate of active L-lysine-α-oxidase producer on the highly infectious Tobacco ringspot virus and revealed anti-viral activity of it when enzyme concentration was at least 1.0 U/ml.

• Authors: Yu A Shneider, I P Smirnova, E V Karimova
• Journal: Bulletin of experimental biology and medicine (Bull Exp Biol Med) Vol. 162 Issue 1 Pg. 79-81 (Nov 2016) ISSN: 1573-8221 [Electronic] United States
• PMID: 27878716 (Publication Type: Journal Article)

Chemical References

• Antiviral Agents
• Culture Media
• Fungal Proteins
• RNA, Viral
• Amino Acid Oxidoreductases
• L-lysine oxidase

Topics

• Amino Acid Oxidoreductases (biosynthesis, isolation & purification, pharmacology)
• Antiviral Agents (isolation & purification, metabolism, pharmacology)
• Balsaminaceae (virology)
• Culture Media (chemistry)
• Fermentation
• Fungal Proteins (biosynthesis, isolation & purification, pharmacology)
• Nepovirus (drug effects, genetics, growth & development)
• RNA, Viral (antagonists & inhibitors, biosynthesis)
• Reverse Transcriptase Polymerase Chain Reaction
• Trichoderma (chemistry, enzymology, growth & development)