Abstract | BACKGROUND: OBJECTIVES: This study sought to establish a mouse model of fish allergy resembling human disease and to investigate whether mouse and rabbit IgG antibodies induced by immunization with a hypoallergenic mutant of the major carp allergen protect against allergic symptoms in sensitized mice. METHODS: C3H/HeJ mice were sensitized with recombinant wildtype Cyp c 1 or carp extract by intragastric gavage. Antibody, cellular immune responses, and epitope specificity in sensitized mice were investigated by ELISA, rat basophil leukemia assay, T-cell proliferation experiments using recombinant wildtype Cyp c 1, and overlapping peptides spanning the Cyp c 1 sequence. Anti-hypoallergenic Cyp c 1 mutant mouse and rabbit sera were tested for their ability to inhibit IgE recognition of Cyp c 1, Cyp c 1-specific basophil degranulation, and Cyp c 1-induced allergic symptoms in the mouse model. RESULTS: A mouse model of fish allergy mimicking human disease regarding IgE epitope recognition and symptoms as close as possible was established. Administration of antisera generated in mice and rabbits by immunization with a hypoallergenic Cyp c 1 mutant inhibited IgE binding to Cyp c 1, Cyp c 1-induced basophil degranulation, and allergic symptoms caused by allergen challenge in sensitized mice. CONCLUSIONS:
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Authors | Raphaela Freidl, Antonia Gstoettner, Ulrike Baranyi, Ines Swoboda, Frank Stolz, Margarete Focke-Tejkl, Thomas Wekerle, Ronald van Ree, Rudolf Valenta, Birgit Linhart |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 139
Issue 6
Pg. 1897-1905.e1
(Jun 2017)
ISSN: 1097-6825 [Electronic] United States |
PMID | 27876628
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Allergens
- Antibodies, Blocking
- Calcium-Binding Proteins
- Cyp c 1.01 protein, carp
- Fish Proteins
- Immune Sera
- Immunoglobulin G
- Parvalbumins
- Immunoglobulin E
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Topics |
- Allergens
(genetics, immunology)
- Animals
- Antibodies, Blocking
(immunology)
- Basophils
(physiology)
- Calcium-Binding Proteins
(genetics, immunology)
- Carps
(immunology)
- Cell Degranulation
- Desensitization, Immunologic
- Disease Models, Animal
- Female
- Fish Proteins
(genetics, immunology)
- Food Hypersensitivity
(genetics, immunology, prevention & control)
- Humans
- Immune Sera
(immunology)
- Immunization
- Immunoglobulin E
(immunology)
- Immunoglobulin G
(immunology)
- Mice, Inbred C3H
- Mutation
- Parvalbumins
(genetics, immunology)
- Rabbits
- Rats
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