A novel method for sensitive microRNA detection: Electropolymerization based doping.

Abstract

In the proposed study, for the first time, sensitive electrochemical detection of a breast cancer biomarker microRNA (miRNA), mir-21 was achieved via electropolymerized polypyrrole (PPy) modified pencil graphite electrodes (PPy/PGE). The detection of hybridization of electrochemically doped probe miRNA, antimir-21, with its complementary target, mir-21 was monitored by either electrochemical impedance spectroscopy (EIS) via comparison of charge transfer resistance (Rct) values before and after hybridization or by electrochemical reduction signal of an hybridization indicator, Meldola's blue (MDB). The study covers all the optimization steps for hybridization procedure and electropolymerization of pyrrole as well as detection from real samples of breast cancer cell line, MCF-7. The designed sensor shows a high selectivity and a low detection limit of 0.17nM thanks to electrical conductivity and porous structure of PPy.

• Authors: Merve Kaplan, Tugba Kilic, Gunnur Guler, Jihane Mandli, Aziz Amine, Mehmet Ozsoz
• Journal: Biosensors & bioelectronics (Biosens Bioelectron) Vol. 92 Pg. 770-778 (Jun 15 2017) ISSN: 1873-4235 [Electronic] England
• PMID: 27836600 (Publication Type: Evaluation Study, Journal Article)
• Copyright: Copyright © 2016. Published by Elsevier B.V.

Chemical References

• MIRN21 microRNA, human
• MicroRNAs
• Polymers
• Pyrroles
• RNA Probes
• polypyrrole
• Graphite

Topics

• Biosensing Techniques (methods)
• Breast Neoplasms (genetics)
• Dielectric Spectroscopy (methods)
• Electrochemical Techniques (methods)
• Electrodes
• Female
• Graphite (chemistry)
• Humans
• Limit of Detection
• MCF-7 Cells
• MicroRNAs (analysis, genetics)
• Nucleic Acid Hybridization (methods)
• Polymerization
• Polymers (chemistry)
• Pyrroles (chemistry)
• RNA Probes (chemistry, genetics)