Abstract | BACKGROUND: METHODS: DBA/1 mice with collagen II-induced arthritis were treated with GMSCs from the C57BL/6 J mouse, the B6Smn.C3-FasLgld/J mouse (FasL-/- GMSCs), and FasL overexpressed FasL-/- GMSCs (FasL TF GMSCs). Inflammation was evaluated by measuring clinical score, tumor necrosis factor (TNF)-α and anti- collagen II antibody levels, and histological analyses. The levels of CD4+ Th cell subsets in spleens and draining lymph nodes were assessed by flow cytometric analysis. RESULTS: Systemic infusion of GMSCs can significantly reduce the severity of experimental arthritis, and resume the balance of Th cell subsets. FasL-/- GMSCs failed to induce apoptosis of activated T cells in vitro and in vivo, and therefore show no therapeutic effects, whereas FasL TF GMSCs can rescue the immunosuppressant effects in the treatment of CIA. CONCLUSIONS: GMSC-based therapy induces T-cell apoptosis via the FasL/Fas pathway and results in immune tolerance and amelioration of the CIA inflammation.
|
Authors | Yongchun Gu, Songtao Shi |
Journal | Arthritis research & therapy
(Arthritis Res Ther)
Vol. 18
Issue 1
Pg. 262
(11 11 2016)
ISSN: 1478-6362 [Electronic] England |
PMID | 27836015
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
|
Topics |
- Animals
- Apoptosis
(immunology)
- Arthritis, Experimental
(immunology, pathology)
- Arthritis, Rheumatoid
(immunology)
- Blotting, Western
- Enzyme-Linked Immunosorbent Assay
- Fas Ligand Protein
(immunology)
- Female
- Flow Cytometry
- Gingiva
(cytology)
- Male
- Mesenchymal Stem Cell Transplantation
(methods)
- Mice
- Mice, Inbred C57BL
- Mice, Inbred DBA
- Mice, Knockout
- T-Lymphocytes
(immunology)
|