[Effect of low-selenium diet on expressions of CCR7, CD206 and CD163 in the liver and kidney of rats].

Abstract

OBJECTIVE:

To investigate the effect of low-selenium diet on the liver and kidneys of rats and explore the role of macrophage polarization into M1 and M2 phenotypes in liver and kidney injuries.

METHODS:

Twenty-four rats (12 female and 12 male) were randomly divided into control group and low-selenium group and fed with normal chow (dietary selenium of 0.18 mg/kg) and low-selenium diet (dietary selenium of 0.02 mg/kg) for 109 days. After the feeding, the rats were sacrificed for HE staining to observe liver and kidney pathologies, and immunohistochemistry was performed for analyzing CCR7, CD206, CD163-positive cell numbers in the liver and kidneys.

RESULTS:

The rats in low-selenium group showed severer fibrosis in the liver and kidney than the control group. In either male or female rats in low-selenium group, CCR7 and CD206 expressions in the liver were comparable with those in control group, but CD163 expression was lower than that in the control group (P<0.05 for both female and male rats). In the kidney, the proximal tubule showed a slightly higher while the distal tubule showed a slightly lower CCR7 expression in low selenium group than in the control group (P>0.05). In low-selenium group, a significantly lower CD163 expression in the distal tubule and a significantly higher CD206 expression in the proximal tubule were noted as compared with the control group (P<0.05 in both female and male rats). Compared with the control rats, the male rats in low-selenium group, but not the female rats, showed a significantly lower CD163 expression in the proximal tubule of the kidney (P<0.05); the female but not the male rats in low-selenium group show a higher CD206 expression in the distal tubule (P<0.05).

CONCLUSION:

Low-selenium diet can cause liver and kidney fibrosis in rats and may inhibit macrophage activation into the M2 phenotype.

• Authors: Li-Yun Wang, Jian-Hua Yi, Hang-Chao Xu, Xiao-Fang Wu, Dan-Yang Li, Jing Han
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 36 Issue 10 Pg. 1357-1363 (Oct 20 2016) ISSN: 1673-4254 [Print] China
• PMID: 27777198 (Publication Type: Journal Article)

Chemical References

• Antigens, CD
• Antigens, Differentiation, Myelomonocytic
• CD163 antigen
• Ccr7 protein, rat
• Lectins, C-Type
• Mannose Receptor
• Mannose-Binding Lectins
• Receptors, CCR7
• Receptors, Cell Surface
• Selenium

Topics

• Animals
• Antigens, CD (metabolism)
• Antigens, Differentiation, Myelomonocytic (metabolism)
• Diet
• Female
• Fibrosis
• Kidney (metabolism, pathology)
• Lectins, C-Type (metabolism)
• Liver (metabolism, pathology)
• Macrophage Activation
• Male
• Mannose Receptor
• Mannose-Binding Lectins (metabolism)
• Rats
• Receptors, CCR7 (metabolism)
• Receptors, Cell Surface (metabolism)
• Selenium (administration & dosage)