Abstract |
The enzyme inhibition by natural and/ or low-cost compounds may represent a valuable adjunct to traditional serotherapy performed in cases of snakebite, mainly with a view to mitigate the local effects of envenoming. The objective of this study was to evaluate possible interactions between vitamins and enzymes that comprise Bothrops atrox and Crotalus durissus terrificus venoms, in vitro. Proteolysis inhibition assays (substrates: azocasein, collagen, gelatin and fibrinogen), hemolysis, coagulation, hemagglutination were carried out using different proportions of vitamins in face of to inhibit minimum effective dose of each venom. The vitamins were responsible for reducing 100% of breaking azocasein by C.d.t. venom, thrombolysis induced by B. atrox and fibrinogenolysis induced by both venoms. It is suggested the presence of interactions between vitamin and the active site of enzymes, for example the interactions between hydrophobic regions present in the enzymes and vitamin E, as well as the inhibitions exercised by antioxidant mechanism.
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Authors | Carlos H M Oliveira, Anderson A Simão, Marcus V C Trento, Pedro H S César, Silvana Marcussi |
Journal | Anais da Academia Brasileira de Ciencias
(An Acad Bras Cienc)
Vol. 88
Issue 3 Suppl
Pg. 2005-2016
( 2016)
ISSN: 1678-2690 [Electronic] Brazil |
PMID | 27737338
(Publication Type: Journal Article)
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Chemical References |
- Crotalid Venoms
- Phospholipase A2 Inhibitors
- Protease Inhibitors
- Vitamins
- Vitamin B Complex
- Vitamin E
- Phospholipases A2
- Peptide Hydrolases
- Ascorbic Acid
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Topics |
- Animals
- Ascorbic Acid
(pharmacology)
- Bothrops
- Crotalid Venoms
(antagonists & inhibitors, enzymology)
- Crotalus
- Peptide Hydrolases
- Phospholipase A2 Inhibitors
(pharmacology)
- Phospholipases A2
- Protease Inhibitors
(pharmacology)
- Vitamin B Complex
(pharmacology)
- Vitamin E
(pharmacology)
- Vitamins
(pharmacology)
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