Surface chemistry from wettability and charge for the control of mesenchymal stem cell fate through self-assembled monolayers.

Abstract

Self-assembled monolayers (SAMs) of alkanethiols on gold are highly controllable model substrates and have been employed to mimic the extracellular matrix for cell-related studies. This study aims to systematically explore how surface chemistry influences the adhesion, morphology, proliferation and osteogenic differentiation of mouse mesenchymal stem cells (mMSCs) using various functional groups (-OEG, -CH3, -PO3H2, -OH, -NH2 and -COOH). Surface analysis demonstrated that these functional groups produced a wide range of wettability and charge: -OEG (hydrophilic and moderate iso-electric point (IEP)), -CH3 (strongly hydrophobic and low IEP), -PO3H2 (moderate wettability and low IEP), -OH (hydrophilic and moderate IEP), -NH2 (moderate wettability and high IEP) and -COOH (hydrophilic and low IEP). In terms of cell responses, the effect of wettability may be more influential than charge for these groups. Moreover, compared to -OEG and -CH3 groups, -PO3H2, -OH, -NH2 and -COOH functionalities tended to promote not only cell adhesion, proliferation and osteogenic differentiation but also the expression of αv and β1 integrins. This finding indicates that the surface chemistry may guide mMSC activities through αv and β1 integrin signaling pathways. Model surfaces with controllable chemistry may provide insight into biological responses to substrate surfaces that would be useful for the design of biomaterial surfaces.

• Authors: Lijing Hao, Xiaoling Fu, Tianjie Li, Naru Zhao, Xuetao Shi, Fuzhai Cui, Chang Du, Yingjun Wang
• Journal: Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 148 Pg. 549-556 (Dec 01 2016) ISSN: 1873-4367 [Electronic] Netherlands
• PMID: 27690244 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Integrins
• Osteocalcin
• Gold

Topics

• Adsorption
• Animals
• Cell Adhesion (physiology)
• Cell Differentiation (genetics, physiology)
• Cell Line
• Cell Proliferation (physiology)
• Extracellular Matrix (chemistry, metabolism)
• Gene Expression
• Gold (chemistry)
• Hydrophobic and Hydrophilic Interactions
• Integrins (genetics, metabolism)
• Mesenchymal Stem Cells (chemistry, cytology, metabolism)
• Mice
• Osteocalcin (genetics, metabolism)
• Osteogenesis (genetics, physiology)
• Photoelectron Spectroscopy
• Reverse Transcriptase Polymerase Chain Reaction
• Spectroscopy, Fourier Transform Infrared
• Surface Properties
• Wettability