Carbonic anhydrase enzymes II, VII, IX and XII in colorectal carcinomas.

Abstract

AIM:

To investigate expression of four alpha-carbonic anhydrases (CAs) in colorectal carcinomas (CRC) and compare the results with patients' survival.

METHODS:

Colorectal carcinoma samples from 539 CRC patients and control tissues were arranged as tissue microarrays and analyzed with antibodies against CA II, CA VII, CA IX, and CA XII. Intensity and extent of staining were both scored from 0 to 3 in each sample. These enzyme expression levels were then correlated to patients' survival and clinicopathological parameters, which were tumor differentiation grade and stage, site of tumor, patients' age, and gender. Kaplan-Meier analysis and Cox regression hazard ratio model were used to analyze survival data.

RESULTS:

CA II and CA XII staining intensities correlated with patients' survival in that higher expression indicated poorer prognosis. In Cox regression analysis one unit increase in the CA II intensity increased the hazard ratio to 1.19 fold (CI: 1.04-1.37, P = 0.009). A significant correlation was also found when comparing CA XII staining intensity with survival of CRC patients (HR = 1.18, 95%CI: 1.01-1.38, P = 0.036). The extent of CA XII immunostaining did not correlate to the patients' survival (P = 0.242, Kaplan-Meier analysis). A significant interaction between age group and extent of the CA II staining was found. Increased extent of CA II had a significant hazard ratio among patients 65 years and older (1.42, 95%CI: 1.16-1.73, P = 0.0006). No correlations were found between CA VII (intensity P = 0.566, extent P = 0.495, Kaplan-Meier analysis), or CA IX (intensity P = 0.879, extent P = 0.315, Kaplan-Meier analysis) immunostaining results and survival, or the other parameters.

CONCLUSION:

The present findings indicate that CA II and CA XII could be useful in predicting survival in CRC.

• Authors: Pia Viikilä, Antti J Kivelä, Harri Mustonen, Selja Koskensalo, Abdul Waheed, William S Sly, Jaromir Pastorek, Silvia Pastorekova, Seppo Parkkila, Caj Haglund
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 22 Issue 36 Pg. 8168-77 (Sep 28 2016) ISSN: 2219-2840 [Electronic] United States
• PMID: 27688658 (Publication Type: Journal Article)

Chemical References

• Antigens, Neoplasm
• Biomarkers, Tumor
• Carbonic Anhydrase II
• CA9 protein, human
• Carbonic Anhydrase IX
• Carbonic Anhydrases
• carbonic anhydrase VI
• carbonic anhydrase XII

Topics

• Aged
• Antigens, Neoplasm (metabolism)
• Biomarkers, Tumor (metabolism)
• Carbonic Anhydrase II (metabolism)
• Carbonic Anhydrase IX (metabolism)
• Carbonic Anhydrases (metabolism)
• Colorectal Neoplasms (enzymology, mortality)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Kaplan-Meier Estimate
• Male
• Middle Aged
• Prognosis
• Proportional Hazards Models
• Regression Analysis
• Tissue Array Analysis
• Treatment Outcome