[Construction of a lentivirus vector expressing wheat germ agglutinin and its infection in human adipose-derived stem cells].

Abstract

OBJECTIVE:

To construct a lentivirus vector carrying wheat germ agglutinin (WGA) and evaluate its ability of tracing WGA in the brain of mice with ischemic brain injury.

METHODS:

WGA gene was inserted into the lentiviral vector Plvx IRES-ZsGreen1 using genetic engineering methods. 293T cells were transfected with the vector and 3 packaging plasmids (RPEV, PRRE, and VSVG) to obtain the recombinant lentivirus for infection of human adipose-derived stem cells (hADSCs). The infected hADSCs were injected into the damaged brain area by in situ injection in a mouse model of middle cerebral artery occlusion (MCAO) and the expression of GFP was traced.

RESULTS:

Immunofluorescence identification detected WGA protein expression in the infected hADSCs, which survived in the infarct area of mice with MCAO.

CONCLUSION:

Packaging WGA gene in lentivirus is a reliable approach to allow efficient neuroanatomical tracing of various cells.

• Authors: Juan Wang, Lian-Guo Zhang, Ning Li, Ming Lei, Yun Ma, Yuan-Hong Peng, Wei-Hua Bian
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 36 Issue 9 Pg. 1242-1246 (08 20 2016) ISSN: 1673-4254 [Print] China
• PMID: 27687658 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Wheat Germ Agglutinins

Topics

• Adipose Tissue (cytology)
• Animals
• Genetic Vectors
• HEK293 Cells
• Humans
• Lentivirus
• Mice
• Plasmids
• Stem Cells (cytology)
• Transfection
• Wheat Germ Agglutinins (genetics, metabolism)