Nano-Cesium for Anti-Cancer Properties: An Investigation into Cesium Induced Metabolic Interference.

Abstract

The use of cesium chloride (CsCl) for cancer therapy ("high pH therapy") has been theorized to produce anticancer properties by raising intracellular pH to induce apoptosis. Although considered as "alternative medicine", little scientific evidence supports this theory. Alternatively, cells have no cesium ion (Cs+) mediated channels for clearance. Thus, such unstable electrochemical distributions have the severe potential to disrupt electrochemical dependent cellular processes, such as glucose cotransporters. Hence, a detailed investigation of pH changing effects and glucose uptake inhibition are warranted as a possible cesium-induced anticancer therapy. We developed and characterized cesium nanoparticles (38 ± 6 nm), termed NanoCs, for nanoparticle-mediated internalization of the ion, and compared its treatment to free CsCl. Our investigations suggest that neither NanoCs nor CsCl drastically changed the intracellular pH, negating the theory. Alternatively, NanoCs lead to a significant decrease in glucose uptake when compared to free CsCl, suggesting cesium inhibited glucose uptake. An apoptosis assay of observed cell death affirms that NanoCs leads tumor cells to initiate apoptosis rather than follow necrotic behavior. Furthermore, NanoCs lead to in vivo tumor regression, where H&E analysis confirmed apoptotic cell populations. Thus, NanoCs performed pH-independent anticancer therapy by inducing metabolic stasis.

• Authors: Enrique A Daza, Santosh K Misra, Aaron S Schwartz-Duval, Ayako Ohoka, Callie Miller, Dipanjan Pan
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 8 Issue 40 Pg. 26600-26612 (Oct 12 2016) ISSN: 1944-8252 [Electronic] United States
• PMID: 27662498 (Publication Type: Journal Article)