Concentrations of synovial fluid biomarkers and the prediction of knee osteoarthritis 16 years after anterior cruciate ligament injury.

Abstract

OBJECTIVE:

To describe the longitudinal patterns of release, and investigate the association between a set of synovial fluid biomarkers at the acute and chronic stage and the development of radiographic knee osteoarthritis (OA) after an anterior cruciate ligament (ACL) injury.

DESIGN:

Synovial fluid was aspirated from the acutely ACL-injured knee within the first 2weeks (acute samples), and yearly (chronic samples) up to 7.5 years after injury in 88 subjects (60% men). Non-injured subjects (n = 12) were used as reference group. Aggrecan, cartilage oligomeric matrix protein (COMP), matrix metalloproteinase (MMP)-3 and tissue inhibitor of metalloproteinase (TIMP)-1 in synovial fluid were quantified by immunoassays. The presence of radiographic tibiofemoral (TF) or patellofemoral (PF) OA [Kellgren and Lawrence (K&L) ≥2] was examined with weight-bearing knee radiography 16 years after the ACL injury.

RESULTS:

The average acute and chronic SF concentrations of COMP and aggrecan were elevated in comparison with the reference group (P < 0.001). The levels of COMP and aggrecan clearly decreased approximately half a year after the ACL injury, and returned to reference values during the 7.5 years of follow-up. Using logistic regression analysis neither acute nor chronic concentrations of the four biomarkers were associated with the development of radiographic knee OA at the 16 year follow-up.

CONCLUSION:

Increased synovial fluid concentrations of aggrecan and COMP was related to knee injury, but acute and chronic synovial fluid concentrations of aggrecan, COMP, MMP-3 and TIMP-1 failed to predict knee OA 16 years after ACL injury.

• Authors: P Neuman, L E Dahlberg, M Englund, A Struglics
• Journal: Osteoarthritis and cartilage (Osteoarthritis Cartilage) Vol. 25 Issue 4 Pg. 492-498 (04 2017) ISSN: 1522-9653 [Electronic] England
• PMID: 27654964 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Aggrecans
• COMP protein, human
• Cartilage Oligomeric Matrix Protein
• TIMP1 protein, human
• Tissue Inhibitor of Metalloproteinase-1
• MMP3 protein, human
• Matrix Metalloproteinase 3

Topics

• Adult
• Aggrecans (metabolism)
• Anterior Cruciate Ligament Injuries (complications, metabolism)
• Cartilage Oligomeric Matrix Protein (metabolism)
• Case-Control Studies
• Female
• Follow-Up Studies
• Humans
• Logistic Models
• Male
• Matrix Metalloproteinase 3 (metabolism)
• Osteoarthritis, Knee (diagnostic imaging, etiology, metabolism)
• Prognosis
• Prospective Studies
• Radiography
• Synovial Fluid (metabolism)
• Tissue Inhibitor of Metalloproteinase-1 (metabolism)
• Young Adult