A rational combination is critical to achieve efficiently synergistic therapeutic efficacy for
tumor treatment. Hence, we designed novel antitumor combinations (T-NPs) by integrating the
tumor vascular and
tumor cells dual-targeting
ligand with antiangiogenesis/
antitumor agents. The truncated bFGF
peptide (tbFGF), which could effectively bind to FGFR1 overexpressed on
tumor neovasculature endothelial cells and
tumor cells, was selected to modify PLGA nanoparticles (D/P-NPs) simultaneously loaded with PEDF gene and
paclitaxel in this study. The obtained T-NPs with better
pharmaceutical properties had elevated cytotoxicity and enhanced expression of PEDF and α-
tubulin on FGFR1-overexpressing cells. The uptake of T-NPs increased in C26 cells, probably mediated by tbFGF via specific recognization of the overexpressed FGFR1. T-NPs dramatically disrupted the tube formation of primary human umbilical vein endothelial cells (HUVECs) and displayed improved antiangiogenic activity in the transgenic zebrafish model and the
alginate-encapsulated
tumor cell model. More importantly, T-NPs achieved a markedly higher antitumor efficacy in the C26
tumor-bearing mice model. The antitumor effect involved the inhibition of
tumor cell proliferation and angiogenesis, induction of apoptosis, and down-regulation of FGFR1. The enhanced antitumor activity of T-NPs probably resulted from the raised distribution in
tumor tissues. In addition, T-NPs had no obvious toxicity as evaluated by weight monitoring, serological/biochemical analyses, and H&E staining. These results revealed that T-NPs, an active targeting gene/chemo-
therapy, indeed had superior antitumor efficacy and negligible side effect, suggesting that this novel combination is a potential
tumor therapy and a new treatment strategy and that the tbFGF modified nanoparticles could be applied to a wide range of
tumor-genetic therapies and/or
tumor-chemical
therapies.