Selenylation modification can enhance immune-enhancing activity of Chuanminshen violaceum polysaccharide.

Abstract

Chuanminshen violaceum polysaccharides (CVPS) were extracted, purified and selenizingly modified. The modification has been achieved by using the HNO3- Na2SeO3 method, and selenizing Chuanminshen violaceum polysaccharides (sCVPS) were evaluated for their physicochemical properties and their potential as adjuvant to modulate cellular and humoral immune responses to hepatitis B subunit vaccine in a mouse model. Our results demonstrated that sCVPS significantly promoted splenocytes proliferation and the production of IL-4 and IFN-γ in vitro. In vivo experiments showed that sCVPS significantly increased the rHBsAg-specific IgG level, IgG subclass (IgG1, IgG2a, and IgG2b) antibody titers, T cells proliferation, levels of IL-4, IL-2, and IFN-γ in CD4 (+)T cells and the level of IFN-γ in CD8(+)T cells. Furthermore, sCVPS increased the activity of natural killer cells and cytotoxic T lymphocytes, thus increasing both cellular and humoral immune responses in vivo. The present data suggest that selenylation of CVPS can significantly improve their immune-enhancing activity both in vitro and in vivo, thus representing a powerful adjuvant for vaccine design.

• Authors: Feng Haibo, Jing Fan, Hongquan Bo, Xi Tian, He Bao, Xiaohua Wang
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 153 Pg. 302-311 (Nov 20 2016) ISSN: 1879-1344 [Electronic] England
• PMID: 27561500 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Ltd. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Cytokines
• Hepatitis B Vaccines
• Immunoglobulin G
• Polysaccharides
• Vaccines, Subunit
• Nitric Acid
• Sodium Selenite

Topics

• Adjuvants, Immunologic (chemistry, pharmacology)
• Animals
• Apiaceae (chemistry)
• Cytokines (immunology)
• Female
• Hepatitis B Vaccines (chemistry, immunology)
• Humans
• Immunity, Cellular (drug effects)
• Immunity, Humoral (drug effects)
• Immunoglobulin G (immunology)
• Killer Cells, Natural (immunology)
• Mice
• Nitric Acid (chemistry)
• Polysaccharides (chemistry, immunology, isolation & purification)
• Sodium Selenite (chemistry)
• T-Lymphocytes, Cytotoxic (immunology)
• Vaccines, Subunit (immunology)