A Deafness- and Diabetes-associated tRNA Mutation Causes Deficient Pseudouridinylation at Position 55 in tRNAGlu and Mitochondrial Dysfunction.

Abstract

Several mitochondrial tRNA mutations have been associated with maternally inherited diabetes and deafness. However, the pathophysiology of these tRNA mutations remains poorly understood. In this report, we identified the novel homoplasmic 14692A→G mutation in the mitochondrial tRNAGlu gene among three Han Chinese families with maternally inherited diabetes and deafness. The m.14692A→G mutation affected a highly conserved uridine at position 55 of the TΨC loop of tRNAGlu The uridine is modified to pseudouridine (Ψ55), which plays an important role in the structure and function of this tRNA. Using lymphoblastoid cell lines derived from a Chinese family, we demonstrated that the m.14692A→G mutation caused loss of Ψ55 modification and increased angiogenin-mediated endonucleolytic cleavage in mutant tRNAGlu The destabilization of base-pairing (18A-Ψ55) caused by the m.14692A→G mutation perturbed the conformation and stability of tRNAGlu An approximately 65% decrease in the steady-state level of tRNAGlu was observed in mutant cells compared with control cells. A failure in tRNAGlu metabolism impaired mitochondrial translation, especially for polypeptides with a high proportion of glutamic acid codons such as ND1, ND6, and CO2 in mutant cells. An impairment of mitochondrial translation caused defective respiratory capacity, especially reducing the activities of complexes I and IV. Furthermore, marked decreases in the levels of mitochondrial ATP and membrane potential were observed in mutant cells. These mitochondrial dysfunctions caused an increasing production of reactive oxygen species in the mutant cells. Our findings may provide new insights into the pathophysiology of maternally inherited diabetes and deafness, which is primarily manifested by the deficient nucleotide modification of mitochondrial tRNAGlu.

• Authors: Meng Wang, Hao Liu, Jing Zheng, Bobei Chen, Mi Zhou, Wenlu Fan, Hen Wang, Xiaoyang Liang, Xiaolong Zhou, Gilbert Eriani, Pingping Jiang, Min-Xin Guan
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 291 Issue 40 Pg. 21029-21041 (Sep 30 2016) ISSN: 1083-351X [Electronic] United States
• PMID: 27519417 (Publication Type: Journal Article)
• Copyright: © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Chemical References

• Mitochondrial Proteins
• RNA, Mitochondrial
• RNA, Transfer, Glu
• Pseudouridine
• RNA

Topics

• Asian People
• Base Pairing
• Cell Line
• China
• Deafness (genetics, metabolism)
• Diabetes Mellitus (genetics, metabolism)
• Female
• Humans
• Male
• Mitochondrial Proteins (biosynthesis, genetics)
• Point Mutation
• Protein Biosynthesis (genetics)
• Pseudouridine (genetics, metabolism)
• RNA (genetics, metabolism)
• RNA, Mitochondrial
• RNA, Transfer, Glu (genetics, metabolism)