Abstract | PURPOSE: METHODS AND MATERIALS: Subcutaneous LLC tumors were established in C57BL/6 mice. Standard RT or PRT was given at 2 Gy/d for a total dose of 20 Gy using a 5 days on, 2 days off schedule to mimic clinical delivery. Radiation-induced tumor microenvironment changes were examined after treatment using flow cytometry and antibody-specific histopathology. Normal tissue effects were measured using noninvasive (18)F-fluorodeoxyglucose positron emission tomography/computed tomography after naïve animals were given whole-lung irradiation to 40 Gy in 4 weeks using the same 2-Gy/d regimens. RESULTS: Over the 2 weeks of therapy, PRT was more effective than SRT at reducing tumor growth rate (0.31 ± 0.02 mm(3)/d and 0.55 ± 0.04 mm(3)/d, respectively; P<.007). Histopathology showed a significant comparative reduction in the levels of Ki-67 (14.5% ± 3%), hypoxia (10% ± 3.5%), vascular endothelial growth factor (2.3% ± 1%), and stromal-derived factor-1α (2.5% ± 1.4%), as well as a concomitant decrease in CD45(+) bone marrow-derived cell (BMDC) migration (7.8% ± 2.2%) after PRT. The addition of AMD3100 also decreased CD45(+) BMDC migration in treated tumors (0.6% ± 0.1%). Higher vessel density was observed in treated tumors. No differences were observed in normal lung tissue after PRT or SRT. CONCLUSIONS: Pulsed RT-treated tumors exhibited slower growth and reduced hypoxia. Pulsed RT eliminated initiation of supportive mechanisms utilized by tumors in low oxygen microenvironments, including angiogenesis and recruitment of BMDCs.
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Authors | Jonathan L Kane, Sarah A Krueger, Alaa Hanna, Thomas R Raffel, George D Wilson, Gerard J Madlambayan, Brian Marples |
Journal | International journal of radiation oncology, biology, physics
(Int J Radiat Oncol Biol Phys)
Vol. 96
Issue 1
Pg. 170-8
(09 01 2016)
ISSN: 1879-355X [Electronic] United States |
PMID | 27511854
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 Elsevier Inc. All rights reserved. |
Topics |
- Animals
- Bone Marrow Cells
(radiation effects)
- Carcinoma, Lewis Lung
(pathology, radiotherapy)
- Cell Line, Tumor
- Cell Movement
(radiation effects)
- Dose-Response Relationship, Radiation
- Male
- Mice, Inbred C57BL
- Neoplasms, Experimental
(pathology, radiotherapy)
- Radiation Dose Hypofractionation
- Treatment Outcome
- Tumor Burden
(radiation effects)
- Tumor Microenvironment
(radiation effects)
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