Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs by Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism.

Abstract

BACKGROUND:

Exogenous glucagon-like peptide 2 (GLP-2) stimulates intestinal adaptation after resection in animal models of pediatric short bowel syndrome (SBS). It is unknown whether the molecular mechanisms of such GLP-2 effects are similar to those of postresection spontaneous adaptation. Using preterm pigs as a model, we hypothesized that GLP-2 treatment would change the intestinal proteome within the first week after resection, relative to individuals not resected or resected without GLP-2 treatment.

MATERIALS AND METHODS:

Two-day-old preterm pigs were subjected to resection of 50% distal small intestine and fed total parenteral nutrition without (SBS) or with GLP-2 infusion (3.5 µg/kg/h, SBS+GLP-2) for 5 days. The proteome of the remnant proximal intestine was compared among the SBS, SBS+GLP-2, and unresected pigs, through gel-based proteomics.

RESULTS:

Thirty-two proteins with differential expression were identified. Ten of these proteins were affected by the resection alone (ie, SBS vs unresected pigs). Five of these resection-responsive proteins and another 22 proteins were affected by GLP-2 infusion (ie, SBS+GLP-2 vs SBS or unresected pigs). Resection alone mainly affected cellular structural proteins, while the added GLP-2 treatment affected proteins involved in protein processing and the metabolism of protein, carbohydrate, and sulphur.

CONCLUSION:

In the first days following resection, proteins affected by resection plus GLP-2 treatment differed markedly from those affected by the spontaneous intestinal adaptation following resection alone. Whether more long-term GLP-2 treatment may affect the intestinal proteome following intestinal resection remains unknown.

• Authors: Pingping Jiang, Andreas Vegge, Thomas Thymann, Jennifer Man-Fan Wan, Per Torp Sangild
• Journal: JPEN. Journal of parenteral and enteral nutrition (JPEN J Parenter Enteral Nutr) Vol. 41 Issue 8 Pg. 1293-1300 (11 2017) ISSN: 1941-2444 [Electronic] United States
• PMID: 27503937 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dietary Carbohydrates
• Dietary Proteins
• Glucagon-Like Peptide 2
• Sulfur

Topics

• Adaptation, Physiological
• Animals
• Animals, Newborn
• Dietary Carbohydrates (metabolism)
• Dietary Proteins (metabolism)
• Disease Models, Animal
• Glucagon-Like Peptide 2 (pharmacology)
• Intestine, Small (drug effects, metabolism)
• Parenteral Nutrition, Total
• Short Bowel Syndrome (surgery)
• Sulfur (metabolism)
• Swine