Abstract | BACKGROUND: METHODS: Protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (3.125-50 mcL/mL) were used for 5-30 min. The viability of protoscoleces was confirmed using the eosin exclusion test (0.1% eosin staining). Further, 48 male NMRI mice were used to determine the acute and sub-acute toxicity of B. persicum essential oil. RESULTS: The obtained results revealed that the B. persicum essential oil at the concentrations of 25 and 50 mcL/mL after 5 min of exposure killed 100% protoscoleces. The mean mortality rate of protoscoleces after 10 min of exposure to the concentration of 12.5 mcL/mL was 100%. Lower concentrations (6.25 and 3.125 mcL/mL) of B. persicum essential oil, however, indicated a delayed protoscolicidal effects. The LD50 value of intra-peritoneal injection of the B. persicum essential oil was 1.96 mL/kg body wt. No significant difference (p > 0.05) was observed in the clinical chemistry and hematologic parameters after oral administrations of B. persicum essential oil at the doses 0.05, 0.1, 0.2, and 4 mL/kg for 14 d. CONCLUSION: Our findings demonstrated the potent scolicidal activity of B. persicum with no significant toxicity; it might be used as a natural scolicidal agent in hydatid cyst surgery.
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Authors | Hossein Mahmoudvand, Razieh Tavakoli Oliaei, Seyed Reza Mirbadie, Farnaz Kheirandish, Amir Tavakoli Kareshk, Behrouz Ezatpour, Hormoz Mahmoudvand |
Journal | Surgical infections
(Surg Infect (Larchmt))
Vol. 17
Issue 6
Pg. 713-719
(Dec 2016)
ISSN: 1557-8674 [Electronic] United States |
PMID | 27501060
(Publication Type: Journal Article)
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Chemical References |
- Anthelmintics
- Oils, Volatile
- Plant Extracts
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Topics |
- Animals
- Anthelmintics
(adverse effects, pharmacology, therapeutic use, toxicity)
- Apiaceae
- Echinococcosis
(drug therapy, parasitology)
- Echinococcus
(drug effects)
- Liver
(parasitology)
- Male
- Mice
- Oils, Volatile
(adverse effects, pharmacology, therapeutic use, toxicity)
- Plant Extracts
(adverse effects, pharmacology, therapeutic use, toxicity)
- Sheep
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