The Effect of TNF-α Blocker HL036337 and Its Best Concentration to Inhibit Dry Eye Inflammation.

Abstract

PURPOSE:

Dry eye syndrome is commonly thought of as an inflammatory disease, and we have previously presented data showing the effectiveness of topical TNF-α blocker agents for the treatment of this condition. The purpose of this study was to investigate the effectiveness of the TNF-α blocking agent HL036337 compared to cyclosporine A for the treatment of dry eye induced inflammation in order to establish whether HL036337 represents a more effective method for suppressing inflammation. The efficacy of HL036337 and cyclosporine A was determined using an experimental murine dry eye model.

METHODS:

The TNF-α blocker HL036337 is a modified form of TNF receptor I. Using dry eye induced C57BL/6 mice (n = 45), corneal erosion was measured at day 4 and 7 after topical treatment with cyclosporine A or HL036337. To determine the effective treatment dose, 0.25, 0.5, 1, 2.5, and 5 mg/mL of HL036337 were topically administered twice per day to dry eye induced murine corneas for 1 week.

RESULTS:

The optimal concentration of the TNF-α blocker HL036337 for treatment of dry eye induced corneal erosion was determined to be 1 mg/mL. Dry eye induced corneal erosion was improved after 1 week with topically applied cyclosporine A and HL036337 at 1 mg/mL.

CONCLUSIONS:

HL036337 administered topically at 1 mg/mL effectively improved corneal erosion induced by dry eye. This finding may also suggest that inhibition of TNF-α can improve dry eye syndrome.

• Authors: Wungrak Choi, Hyemi Noh, Areum Yeo, Hanmil Jang, Hyea Kyung Ahn, Yeon Jung Song, Hyung Keun Lee
• Journal: Korean journal of ophthalmology : KJO (Korean J Ophthalmol) Vol. 30 Issue 4 Pg. 302-8 (Aug 2016) ISSN: 2092-9382 [Electronic] Korea (South)
• PMID: 27478358 (Publication Type: Journal Article)

Chemical References

• Ophthalmic Solutions
• Tumor Necrosis Factor-alpha

Topics

• Animals
• Cornea (diagnostic imaging)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Dry Eye Syndromes (diagnosis, drug therapy)
• Female
• Mice
• Mice, Inbred C57BL
• Microscopy, Acoustic
• Ophthalmic Solutions (administration & dosage)
• Tumor Necrosis Factor-alpha (antagonists & inhibitors)