Applications of a Rapid and Sensitive Dengue DUO Rapid Immunochromatographic Test Kit as a Diagnostic Strategy during a Dengue Type 2 Epidemic in an Urban City.

Abstract

Dengue infection is a major health problem in tropical and subtropical countries. A prospective observational study in a university-affiliated hospital was conducted between August 2015 and September 2015. Patients who visited the emergency department (ED) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (NS1), IgM/IgG rapid immunochromatographic tests and real-time polymerase chain reaction (RT-PCR) to evaluate the performance of the rapid tests. Considering the RT-PCR as the gold standard for the dengue diagnosis, the ideal primary results of sensitivity (80-100%), specificity (60-84%), positive predicted value(75%-95%), and negative predicted value (70-100%) suggested that the NS1-based test with or without a combination of IgM and IgG tests have good diagnostic performances in detecting dengue infections, even in the afebrile or elderly populations.

• Authors: Hsin-I Shih, Hsiang-Chin Hsu, Chi-Jung Wu, Chih-Hao Lin, Chia-Ming Chang, Yi-Fang Tu, Chih-Chia Hsieh, Chih-Hsien Chi, Tzu-Ching Sung
• Journal: PloS one (PLoS One) Vol. 11 Issue 7 Pg. e0158437 ( 2016) ISSN: 1932-6203 [Electronic] United States
• PMID: 27415767 (Publication Type: Journal Article, Observational Study)

Chemical References

• Immunoglobulin G
• Immunoglobulin M
• RNA, Viral
• Reagent Kits, Diagnostic

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Child
• Child, Preschool
• Chromatography, Affinity (methods)
• Cities
• Dengue (diagnosis, epidemiology)
• Epidemics
• Female
• Humans
• Immunoglobulin G (analysis)
• Immunoglobulin M (analysis)
• Infant
• Infant, Newborn
• Male
• Middle Aged
• Prospective Studies
• RNA, Viral (analysis)
• Reagent Kits, Diagnostic
• Real-Time Polymerase Chain Reaction (methods)
• Sensitivity and Specificity
• Young Adult