An ultra-high-performance liquid chromatography-tandem mass spectrometric method for the determination of hederacoside C, a drug candidate for respiratory disorder, in rat plasma.

Abstract

Hederacoside C is a principal bioactive pharmaceutical ingredient of Hedera helix leaf extracts. H. helix extracts have long been used in folk medicine for the treatment of respiratory disorders. Currently, hederacoside C is investigated as a promising candidate for the treatment of respiratory diseases. In this study, an accurate, sensitive, rapid, and reliable bioanalytical method was developed for the determination of hederacoside C in rat plasma using ultra high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). For sample preparation, plasma proteins were precipitated with 0.1% acetic acid in acetonitrile. Waters UPLC BEH C18 (2.1mm I.D.×100mm, 1.7μm) column was used for chromatographic separation. A gradient elution of mobile phases consisting of 0.02% acetic acid in distilled water (solvent A) and 0.02% acetic acid in acetonitrile (solvent B) was used at a flow rate of 0.3mL/min. The multiple reaction monitoring (MRM) mode was used for mass spectrometric detection; the MRM transitions were m/z 1219.7→m/z 469.2 for hederacoside C and m/z 1108.3→m/z 221.2 for ginsenoside Rb1 (internal standard) in the negative ionization mode. A calibration curve was constructed in the range of 10-1000ng/mL. The intra- and inter-day precision and accuracy were within 5%. The developed UPLC-MS/MS method was successfully applied in a pharmacokinetic study of hederacoside C in rats. Hederacoside C was quickly but inadequately absorbed from the gastrointestinal tract of rats resulting in extremely low bioavailability and relatively slow clearance.

• Authors: Shaheed Ur Rehman, Min Sun Choi, In Sook Kim, Seung Hyun Kim, Hye Hyun Yoo
• Journal: Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 129 Pg. 90-95 (Sep 10 2016) ISSN: 1873-264X [Electronic] England
• PMID: 27411171 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Plant Extracts
• hederacoside C
• Oleanolic Acid

Topics

• Animals
• Chromatography, High Pressure Liquid (methods)
• Hedera
• Male
• Oleanolic Acid (administration & dosage, analogs & derivatives, blood, isolation & purification)
• Plant Extracts (administration & dosage, blood, isolation & purification)
• Rats
• Rats, Sprague-Dawley
• Respiration Disorders (blood, drug therapy)
• Tandem Mass Spectrometry (methods)