Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: MATERIALS AND METHODS: Primary stromal cells derived from ectopic endometriotic lesions of patients with endometriosis were isolated and cultured. The inhibition effect of WXT on cell proliferation was determined by MTT. Apoptosis of ectopic endometriotic cells treated with WXT was analyzed with Annexin V-FITC/7-AAD staining. The activation of caspases was detected by western blot analysis. The influence of WXT on migration of ectopic endometriotic cells was measured by scratch wound healing assay and Transwell assay. The DNA binding activity of NF-κB and the expression of nuclear p65 protein were determined by electrophoretic mobility shift assay and western blot analysis, respectively. The impact of WXT on the expression of NF-κB regulated gene products involved in apoptosis and migration was determined by western blot analysis. RESULTS: WXT inhibited the proliferation of ectopic endometriotic cells in a time- and dose-dependent manner. In addition, WXT treatment resulted in significant induction of apoptosis through the activation of caspases and inhibition of migration in ectopic endometriotic cells. WXT notably suppressed constitutive NF-κB- DNA-binding activity as well as TNF-α induced nuclear translocation of NF-κB p65 subunit in ectopic endometriotic cells. Moreover, WXT diminished the expression of NF-κB regulated gene products involved in apoptosis and migration, including c-IAP1, c-IAP2, XIAP, survivin, Mcl-1, COX-2 and MMP-9. CONCLUSIONS: Our results indicate that WXT induces apoptosis and inhibits migration of ectopic endometriotic stromal cells.
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Authors | Zhenzhen Zhang, Xiaolan Cheng, Tao Gui, Jia Tao, Meihua Huang, Li Zhu, Mei Luo, Peng Cao, Guiping Wan |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 194
Pg. 386-394
(Dec 24 2016)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 27401290
(Publication Type: Journal Article)
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Copyright | Copyright © 2016. Published by Elsevier Ireland Ltd. |
Chemical References |
- Drugs, Chinese Herbal
- NF-kappa B
- wenshen xiaozheng tang
|
Topics |
- Apoptosis
(drug effects)
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Drugs, Chinese Herbal
(pharmacology)
- Endometriosis
(metabolism, pathology)
- Female
- Humans
- NF-kappa B
(metabolism)
- Stromal Cells
(drug effects, metabolism, pathology)
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