Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: The effect of a standardised aqueous extract of the stem bark of Terminalia arjuna (Roxb.) in preventing monocrotaline (MCT)-induced PH in rat was investigated. MATERIALS AND METHODS: The study was approved by Institutional Animal Ethics Committe. Male Wistar rats (150-200g) were randomly distributed into five groups; Control, MCT (50mg/kg subcutaneously once), sildenafil (175µg/kg/day three days after MCT for 25 days), and Arjuna extract (TA125 and TA250 mg/kg/day orally after MCT for 25 days). PH was confirmed by right ventricular weight to left ventricular plus septum weight (Fulton index), right ventricular systolic pressure (RVSP), echocardiography, percentage medial wall thickness of pulmonary arteries (%MWT). Oxidative stress in lung was assessed by super oxide dismutase (SOD), catalase, reduced glutathione (GSH) and thiobarbituric acid reactive substance ( TBARS). The protein expressions of nicotinamide adenine dinucleotide phosphate ( NADPH) oxidase (NOX-1) in lung and gene expression of Bcl2 and Bax in heart were analyzed by Western blot and RT PCR respectively. RESULTS: MCT caused right ventricular hypertrophy (0.58±0.05 vs 0.31±0.05; P<0.001 vs. control) and increase in RVSP (33.5±1.5 vs 22.3±4.7mm of Hg; P<0.001). Both sildenafil and Arjuna prevented hypertrophy and RVSP. Pulmonary artery acceleration time to ejection time ratio in echocardiography was decreased in PH rats (0.49±0.05 vs 0.32±0.06; P<0.001) which was prevented by sildenafil (0.44±0.06; P<0.01) and TA250 (0.45±0.06; P<0.01). % MWT of pulmonary arteries was increased in PH and was prevented by TA250. Increase in TBARS (132.7±18.4 vs 18.8±1.6nmol/mg protein; P<0.001) and decrease in SOD (58.4±14.1 vs 117.4±26.9U/mg protein; P<0.001) and catalase (0.30±0.05 vs 0.75±0.31U/mg protein; P<0.001) were observed in lung tissue of PH rats, which were prevented by sildenafil and both the doses of Arjuna extract. Protein expression of NOX1 was significantly increased in lung and gene expression of Bcl2/Bax ratio was significantly decreased in right ventricle in MCT-induced PH, both were significantly prevented by Arjuna and sildenafil. CONCLUSIONS: Aqueous extract of Terminalia arjuna prevented MCT-induced pulmonary hypertension which may be attributed to its antioxidant as well as its effects on pulmonary arteriolar wall thickening.
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Authors | Himanshu Meghwani, Pankaj Prabhakar, Soheb A Mohammed, Sandeep Seth, Milind P Hote, Sanjay K Banerjee, Sudheer Arava, Ruma Ray, Subir Kumar Maulik |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 197
Pg. 184-194
(Feb 02 2017)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 27401289
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Antihypertensive Agents
- Antioxidants
- Bcl2 protein, rat
- Plant Extracts
- Proto-Oncogene Proteins c-bcl-2
- bcl-2-Associated X Protein
- Water
- Sildenafil Citrate
- Catalase
- NADH, NADPH Oxidoreductases
- NADPH Oxidase 1
- NOX1 protein, rat
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Topics |
- Animals
- Antihypertensive Agents
(chemistry, pharmacology)
- Antioxidants
(chemistry, pharmacology)
- Catalase
(metabolism)
- Disease Models, Animal
- Heart Ventricles
(drug effects, metabolism)
- Hypertension, Pulmonary
(drug therapy, metabolism)
- Hypertrophy, Right Ventricular
(drug therapy, metabolism)
- Lung
(drug effects, metabolism)
- Male
- Medicine, Ayurvedic
- NADH, NADPH Oxidoreductases
(metabolism)
- NADPH Oxidase 1
- Plant Bark
(chemistry)
- Plant Extracts
(chemistry, pharmacology)
- Plant Stems
(chemistry)
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- Pulmonary Artery
(drug effects, metabolism)
- Rats
- Rats, Wistar
- Sildenafil Citrate
- Terminalia
(chemistry)
- Water
(chemistry)
- bcl-2-Associated X Protein
(metabolism)
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