Immunoproteomic studies on paediatric opsoclonus-myoclonus associated with neuroblastoma.

Abstract

We aimed to identify new cell-membrane antigens implicated in opsoclonus-myoclonus with neuroblastoma. The sera of 3 out of 14 patients showed IgG electron-microscopy immunogold reactivity on SH-SY5Y neuroblastoma cells. Immunoprecipitation experiments using rat brain synaptosomes and SH-SY5Y cells led to the identification of: (1) thirty-one nuclear/cytoplasmic proteins (including antigens HuB, HuC); (2) seven neuronal membrane proteins, including the Shaw-potassium channel Kv3.3 (KCNC3), whose genetic disruption in mice causes ataxia and generalized muscle twitching. Although cell-based assays did not demonstrate direct antigenicity, our findings point to Shaw-related subfamily of the potassium voltage-gated channels complexed proteins as hypothetical antigenic targets.

• Authors: Estefanía Torres-Vega, María Durán-Moreno, Manuel Sánchez Del Pino, Yania Yáñez, Adela Cañete, Victoria Castel, Rogelio López-Cuevas, Juan Jesús Vílchez, Josep Dalmau, Francesc Graus, José Manuel García Verdugo, Luis Bataller
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 297 Pg. 98-102 (08 15 2016) ISSN: 1872-8421 [Electronic] Netherlands
• PMID: 27397082 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Cell Adhesion Molecules, Neuronal
• IgLON5 protein, human
• Membrane Proteins
• Nerve Tissue Proteins
• Nuclear Proteins
• Shaw Potassium Channels

Topics

• Animals
• Brain Neoplasms (complications, immunology, pathology)
• Cell Adhesion Molecules, Neuronal (metabolism)
• Cell Line, Tumor
• Central Nervous System (metabolism, ultrastructure)
• Child
• Databases, Factual (statistics & numerical data)
• Encephalitis (complications, immunology)
• Female
• HEK293 Cells
• Humans
• Male
• Membrane Proteins (metabolism)
• Nerve Tissue Proteins (metabolism, ultrastructure)
• Neuroblastoma (complications, immunology, pathology)
• Nuclear Proteins (metabolism, ultrastructure)
• Opsoclonus-Myoclonus Syndrome (complications, immunology, pathology)
• Rats
• Rats, Wistar
• Shaw Potassium Channels (immunology, metabolism, ultrastructure)
• Synaptosomes (metabolism, ultrastructure)
• Thymoma (complications)