Abstract |
Gamabufotalin (CS-6) is a major bufadienolide of Chansu, which shows desirable metabolic stability and less adverse effect in cancer therapy. CS-6 treatment inhibited the proliferation of NSCLC in a nanomolar range. And CS-6 could induce G2/M cell cycle arrest and apoptosis in A549 cells. However, its molecular mechanism in antitumor activity remains poorly understood. We employed a quantitative proteomics approach to identify the potential cellular targets of CS-6, and found 38 possible target-related proteins. Among them, 31 proteins were closely related in the protein-protein interaction network. One of the regulatory nodes in key pathways was occupied by Hsp90. Molecular docking revealed that CS-6 interacted with the ATP-binding sites of Hsp90. In addition, CS-6 inhibited the chaperone function of Hsp90 and reduced expression of Hsp90-dependent client proteins. Moreover, CS-6 markedly down-regulated the protein level of Hsp90 in tumor tissues of the xenograft mice. Taken together, our results suggest that CS-6 might be a novel inhibitor of Hsp90, and the possible network associated with CS-6 target-related proteins was constructed, which provided experimental evidence for the preclinical value of using CS-6 as an effective antitumor agent in treatment of NSCLC.
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Authors | Liyuan Zhang, Zhenlong Yu, Yan Wang, Xiaobo Wang, Lianru Zhang, Chao Wang, Qingxi Yue, Xun Wang, Sa Deng, Xiaokui Huo, Xiangge Tian, Shanshan Huang, Baojing Zhang, Xiaochi Ma |
Journal | Oncotarget
(Oncotarget)
Vol. 7
Issue 47
Pg. 76551-76564
(Nov 22 2016)
ISSN: 1949-2553 [Electronic] United States |
PMID | 27384878
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Bufanolides
- HSP90 Heat-Shock Proteins
- gamabufotalin
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Topics |
- Animals
- Antineoplastic Agents
(chemistry, pharmacology)
- Bufanolides
(chemistry, pharmacology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Chromatography, Liquid
- HSP90 Heat-Shock Proteins
(antagonists & inhibitors, chemistry, metabolism)
- Humans
- Lung Neoplasms
(metabolism)
- Mice
- Models, Molecular
- Molecular Conformation
- Proteomics
(methods)
- Quantitative Structure-Activity Relationship
- Tandem Mass Spectrometry
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