The study was designed to evaluate the effects of hydromethanolic extract of tuberous root of M. jalapa and its
terpenoid and
flavonoid fractions on cutaneous wound healing in Wistar Albino rats. The hydromethanolic extract was subfractionated by sequential extraction in
solvents (moderately nonpolar to polar). The extract and its (
terpenoid and
flavonoid) fractions were used for cutaneous wound healing studies by using excision
wound model on rat. Their effects on
wound contraction rate, biochemical and histological changes, and expression of
growth factors such as
collagen 3A,
basic fibroblast growth factor, and
vascular endothelial growth factor were investigated. The results indicated that
flavonoid treated group showed significant decrease (P < 0.05) in
antioxidant enzyme level as compared to control in wound healing process, whereas
terpenoid fraction showed significant increase (P < 0.05) in expression of
growth factor levels but regeneration and remodeling stages were delayed due to formation of thicker ulcus layer and also there were no hair follicle-like blood capillaries formation which ultimately may lead to formation of
hypertrophic scar of
wound. Therefore, from this study, it can be concluded that
terpenoid fraction prolongs proliferation phase and hence may have tendency to convert the
wound into hypertrophic
wound.