Novel imatinib-loaded silver nanoparticles for enhanced apoptosis of human breast cancer MCF-7 cells.

Abstract

In the current study, in vitro biological feature of imatinib-loaded silver nanoparticles (IMAB-AgNPs) on human breast cancer cell line was investigated. The formation of synthesized silver nanoparticles (AgNPs) was characterized by UV-Visible spectroscopy, EDS, TEM imaging, SEM, FTIR, DLS and Zeta potentiometer. The developed IMAB-AgNPs with maximum percentage of loading efficiency was demonstrated in the average of 130 nm and mostly spherical. Additionally, in vitro drug release study showed a slow and continuous release of imatinib over a period of 80 h. We demonstrated that the synthesized IMAB-AgNPs exhibited a dose-dependent cytotoxicity against MCF-7 cell line. Then, real-time PCR method was also applied for the investigation of Bax and Bcl-2 gene expression in the cells. Comparing IMAB-AgNPs to AgNPs and Imatinib revealed the ability of IMAB-AgNPs to up-regulating Bax/Bcl-2 ratio. An induction of apoptosis was evidenced by Annexin-V/PI detection assay. Based on the current obtained data, the IMAB-AgNPs can exhibit inhibitory effect on viability through up regulation of apoptosis in MCF-7 cancer cells, which provides influencing evidence for the green synthesized AgNPs as a promising sustained drug delivery system.

• Authors: Seyed Ataollah Sadat Shandiz, Mehdi Shafiee Ardestani, Delavar Shahbazzadeh, Artin Assadi, Reza Ahangari Cohan, Vahid Asgary, Soheil Salehi
• Journal: Artificial cells, nanomedicine, and biotechnology (Artif Cells Nanomed Biotechnol) Vol. 45 Issue 6 Pg. 1-10 (Sep 2017) ISSN: 2169-141X [Electronic] England
• PMID: 27362495 (Publication Type: Journal Article)

Chemical References

• BAX protein, human
• BCL2 protein, human
• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein
• Silver
• Imatinib Mesylate

Topics

• Apoptosis (drug effects)
• Breast Neoplasms (drug therapy, metabolism, pathology)
• Drug Delivery Systems (methods)
• Female
• Humans
• Imatinib Mesylate (chemistry, pharmacology)
• MCF-7 Cells
• Metal Nanoparticles (chemistry, therapeutic use)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Silver (chemistry, pharmacology)
• bcl-2-Associated X Protein (metabolism)