Interferon Lambda-3 rs12979860 Variants and Response to Pegylated Interferon in Chronic Hepatitis-C Genotype-3.

Abstract

OBJECTIVE:

To assess the role of single nucleotide polymorphisms (SNPs) near the interferon lambda-3 (IFNλ3) (formal IL-28B) gene rs12979860 in predicting sustained virologic response (SVR) in hepatitis-C virus genotype-3 (HCV-3).

STUDY DESIGN:

Descriptive, analytical study.

PLACE AND DURATION OF STUDY:

Department of Medicine, The Aga Khan University Hospital, Karachi, from July 2012 to June 2014.

METHODOLOGY:

Patients with HCV-3 were classified as sustained virologic response (SVR), relapsers and non-responders. SNPrs12979860 was determined by PCR-RFLPprotocol. Differences between categorical variables were assessed by chi-square or Fisher's exact test, while those between continuous variables were evaluated using the Mann-Whitney U-test. Binary logistic regression analysis by forward conditional method was performed by using significant variables with p-values less than 0.05 as the criteria for model inclusion.

RESULTS:

Out of 115 patients, rs12979860 genotype-CC, CT, TTwas found in 37 (32.2%), 70 (60.9%), and 8 (7%) patients. 72 patients were male with median age of 45 years. Cirrhosis was present in 32 patients. Patients with response failures (no response and relapse, n=36 and 29, respectively) had higher baseline gamma glutamyl transferase (GGT) level (p < 0.001), higher alanine aminotransferase (p=0.027) and cirrhosis (p=0.001) than patients with SVR. Genotype-CC was present in 16/65 in response failures compared to 21/50 who achieved SVR (p=0.048). Rapid virologic response (RVR) (p < 0.001), low GGT(p=0.001) and absence of cirrhosis (p=0.039) were the independent predictive factors for SVR. In patients who could not achieve RVR and in patients with cirrhosis, SVR was seen more in with genotype-CC (p=0.007 and 0.038).

CONCLUSION:

In patients infected with HCV-3, IFNλ3 rs12979860, SNPhas less impact on SVR.

• Authors: Zaigham Abbas, Javed Yakoob, Muhammad A Umer, Ali A Azeem, Saeed S Hamid, Wasim Jafri
• Journal: Journal of the College of Physicians and Surgeons--Pakistan : JCPSP (J Coll Physicians Surg Pak) Vol. 26 Issue 6 Pg. 481-5 (Jun 2016) ISSN: 1681-7168 [Electronic] Pakistan
• PMID: 27353984 (Publication Type: Journal Article)

Chemical References

• Antiviral Agents
• interferon-lambda, human
• Interferon-alpha
• Interleukins
• RNA, Viral
• Interferons
• gamma-Glutamyltransferase
• Alanine Transaminase

Topics

• Adult
• Aged
• Alanine Transaminase (blood, genetics)
• Antiviral Agents (therapeutic use)
• Female
• Genotype
• Hepacivirus (genetics)
• Hepatitis C, Chronic (drug therapy)
• Humans
• Interferon-alpha (therapeutic use)
• Interferons
• Interleukins (genetics)
• Liver Cirrhosis (virology)
• Male
• Middle Aged
• Polymorphism, Restriction Fragment Length
• Polymorphism, Single Nucleotide
• RNA, Viral (blood)
• Treatment Outcome
• gamma-Glutamyltransferase (blood)