Abstract | OBJECTIVE: METHODS: A total of 108 patients with normal cervical (NC), 142 cases of cervical intraepithelial neoplasia (CIN1, n=72; CIN2+, n=70), and 100 new cases of cervical squamous cell carcinoma (SCC) were chosen from the Shanxi Tumor Hospital, Second Hospital of Shanxi Medical University, Maternal and Child Health Center in Taiyuan and Jiexiu during September 2009 and March 2011. HPV16 was detected by multiple PCR. FHIT methylation and protein expression levels were detected by methylation specific PCR (MSP) and Western Blot, respectively. All the data were performed with SPSS 20.0 statistical software. Differences among groups were assessed by Chi-square and Kruskal-Wallis tests. The interaction effects were evaluated by additive model. RESULTS: The prevalence rates of HPV16 infection in CIN1 (45.8%), CIN2+(68.6%) and SCC (73.0%) were significantly higher than that in NC (28.7%, P<0.001). In NC, CIN1, CIN2+ and SCC groups, the FHIT gene methylation rates were 3.7%, 13.9%, 21.4% and 38.0% while the protein expression levels were 1.255±0.130, 1.184±0.172, 1.133±0.126 and 1.099±0.148, respectively. Differences among the groups were statistical significant (P<0.001). With increasing degrees of cervical lesions, the HPV16 infection rate (χ(2)=47.623, P<0.001), FHIT methylation rate (χ(2)=40.147, P<0.001) and the rate of FHIT protein low expression (χ(2)=65.098, P<0.001) were all gradually increasing. There appeared positive additive interaction between FHIT methylation, FHIT protein low expression and infection of HPV16. CONCLUSION:
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Authors | X Z Liu, J Nan, J Li, Y Li, L Ding, J T Wang |
Journal | Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi
(Zhonghua Liu Xing Bing Xue Za Zhi)
Vol. 37
Issue 6
Pg. 858-62
(Jun 2016)
ISSN: 0254-6450 [Print] China |
PMID | 27346116
(Publication Type: Journal Article)
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Chemical References |
- Neoplasm Proteins
- fragile histidine triad protein
- Histidine
- Acid Anhydride Hydrolases
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Topics |
- Acid Anhydride Hydrolases
- Carcinoma, Squamous Cell
- DNA Methylation
- Female
- Histidine
- Human papillomavirus 16
- Humans
- Neoplasm Proteins
- Papillomavirus Infections
- Polymerase Chain Reaction
- Uterine Cervical Neoplasms
- Uterine Cervical Dysplasia
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