Shorter telomeres in adults with Type 1 diabetes correlate with diabetes duration, but only weakly with vascular function and risk factors.

Abstract

OBJECTIVE:

To determine if white blood cell (WBC) telomeres are shorter in Type 1 diabetes (T1D) than in subjects without diabetes (non-DB), and shorter in T1D subjects with vs. without vascular complications; and to determine associations with vascular biomarkers.

RESEARCH DESIGN AND METHODS:

WBC relative telomere length (RTL) was determined by quantitative PCR in a cross-sectional study of 140 non-DB and 199 T1D adults, including 128 subjects without vascular complications (T1DNoCx) and 71 subjects with vascular complications (T1DCx). Relationships of RTL with age, T1D duration, arterial elasticity, pulse pressure and vascular risk factors were determined.

RESULTS:

RTL did not differ by gender within T1D and non-DB groups. Age-adjusted RTL was shorter in T1D vs. non-DB subjects (1.48±0.03 AU vs. 1.64±0.04 AU, p=0.002), but did not differ by T1D complication status (T1DNoCX 1.50±0.04 vs. T1DCX 1.46±0.05, p=0.50), nor correlate with arterial elasticity. Univariate analysis in T1D showed RTL correlated (inversely) with age r=-0.27, p=0.0001, T1D duration r=-0.16, p=0.03, and pulse pressure (r=-0.15, p=0.04), but not with HbA1c, BP, renal function (serum creatinine, ACR, eGFR), lipids, insulin sensitivity, inflammation (CRP, CAMs) or oxidative stress (OxLDL, OxLDL/LDL-C, MPO, PON-1). Multiple regression analysis showed independent determinants of RTL were age and T1D presence (r=0.29, p<0.0001).

CONCLUSIONS:

In this cross-sectional study telomeres were shorter in T1D. RTL correlated inversely with T1D duration, but did not differ by complication status and weakly correlated with pulse pressure and vascular risk factors. Only age and T1D were independent determinants of RTL. Longitudinal studies are merited.

• Authors: Andrzej S Januszewski, Surya S Sutanto, Susan McLennan, David N O'Neal, Anthony C Keech, Stephen M Twigg, Alicia J Jenkins
• Journal: Diabetes research and clinical practice (Diabetes Res Clin Pract) Vol. 117 Pg. 4-11 (Jul 2016) ISSN: 1872-8227 [Electronic] Ireland
• PMID: 27329016 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Biomarkers

Topics

• Adult
• Biomarkers (blood)
• Blood Pressure
• Case-Control Studies
• Cross-Sectional Studies
• Diabetes Mellitus, Type 1 (blood, complications, genetics)
• Diabetic Angiopathies (diagnosis, etiology)
• Female
• Humans
• Inflammation (diagnosis, etiology)
• Insulin Resistance
• Male
• Oxidative Stress
• Real-Time Polymerase Chain Reaction
• Risk Factors
• Telomere (genetics)
• Telomere Shortening (genetics)