Sesamol induces mitochondrial apoptosis pathway in HCT116 human colon cancer cells via pro-oxidant effect.

Abstract

AIMS:

Sesamol lignan is a phenolic compound found in sesame seeds. We investigated the effect of different concentrations of sesamol on oxidative stress in colorectal carcinoma cells (HCT116).

MAIN METHODS:

Antioxidation in vitro was determined from elimination of the DPPH radical, ferric reducing-antioxidant power (FRAP), O2(-), and peroxyl radical scavenging activity. Intracellular O2(-), H2O2 and GSH levels were determined by DHE, DCFH-DA, and CMF-DA assay, respectively. Cell viability was detected by neutral red assay. Cell cycle proportion and mode of apoptotic HCT116 cells death was analyzed by flow cytometry. Apoptosis in sesamol-treated HCT116 cells was confirmed by morphological changes in the nuclei using DAPI staining and changes in mitochondrial membrane potential using the DiOC6(3) assay.

KEY FINDINGS:

Sesamol at both low (0.05 and 0.25mM) and high (0.5, 2, 5, and 10mM) concentrations concurrently reduced FRAP reagent and scavenged DPPH, and O2(-). Sesamol at low concentrations scavenged ROO, but ROO-scavenging was decreased at higher concentrations. Sesamol suppressed cell viability via disruption of cell cycle progression at high concentrations (0.5, 1, 2, and 5mM), thereby causing S-phase arrest and inducing apoptosis-through the production of intracellular O2(-), mitochondrial dysfunction, and DNA fragmentation.

SIGNIFICANCE:

High concentrations of sesamol induced the mitochondrial apoptosis pathway in human colon cancer HCT116 cells via a pro-oxidant effect.

• Authors: Munthipha Khamphio, Sahapat Barusrux, Natthida Weerapreeyakul
• Journal: Life sciences (Life Sci) Vol. 158 Pg. 46-56 (Aug 01 2016) ISSN: 1879-0631 [Electronic] Netherlands
• PMID: 27328416 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Benzodioxoles
• Phenols
• Reactive Oxygen Species
• sesamol

Topics

• Apoptosis (drug effects)
• Benzodioxoles (pharmacology)
• Cell Cycle (drug effects)
• Colonic Neoplasms (pathology)
• HCT116 Cells
• Humans
• Mitochondria (drug effects)
• Phenols (pharmacology)
• Reactive Oxygen Species (pharmacology)