Temporal Profiles of Stress Protein Inductions after Focal Transient Ischemia in Mice Brain.

Abstract

BACKGROUND:

Stress proteins have been found to play important protective roles against ischemic brain injury under hypoxic, oxidative, heat shock, and proteasome stresses.

METHODS:

In the present study, we investigated the temporal profiles of the major stress proteins including hypoxia-inducible factor-1α (HIF-1α), glutathione (GSH), heat shock protein 72 (HSP72), constitutive heat shock cognate protein 73 (HSC73), and ubiquitin after 45 minutes of transient middle cerebral artery occlusion (tMCAO) in the mice brain up to 7 days after reperfusion.

RESULTS:

Immunohistochemical analyses of HIF-1α, GSH, HSP72, and ubiquitin showed little immunoreactivity of neural cells in sham control brain, whereas HSC73 showed a constitutive immunoreactivity. After tMCAO, HSC73 showed the fastest increase at 12 hours in the peri-ischemic area, followed by HIF-1α with a peak at 24 hours, GSH, HSP72, and ubiquitin with a peak at 72 hours. All these stress proteins returned toward the baseline levels until 7 days. In the ischemic core, these stress proteins showed a similar change with less reaction compared to the peri-ischemic area.

CONCLUSIONS:

These data showed temporal expressions of HIF-1α, GSH, HSP72, HSC73, and ubiquitin in the mice brain after tMCAO, which might provide a better understanding of neuroprotective mechanisms and novel targets for therapeutic intervention of brain ischemic disease.

• Authors: Qian Li, Yumiko Nakano, Jingwei Shang, Yasuyuki Ohta, Kota Sato, Mami Takemoto, Nozomi Hishikawa, Toru Yamashita, Koji Abe
• Journal: Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association (J Stroke Cerebrovasc Dis) Vol. 25 Issue 10 Pg. 2344-51 (Oct 2016) ISSN: 1532-8511 [Electronic] United States
• PMID: 27318648 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.

Chemical References

• HSC70 Heat-Shock Proteins
• HSP72 Heat-Shock Proteins
• Heat-Shock Proteins
• Hif1a protein, mouse
• Hspa8 protein, mouse
• Hypoxia-Inducible Factor 1, alpha Subunit
• Ubiquitin
• Glutathione

Topics

• Animals
• Brain (metabolism, pathology)
• Disease Models, Animal
• Glutathione (metabolism)
• HSC70 Heat-Shock Proteins (metabolism)
• HSP72 Heat-Shock Proteins (metabolism)
• Heat-Shock Proteins (metabolism)
• Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
• Immunohistochemistry
• Infarction, Middle Cerebral Artery (metabolism, pathology)
• Ischemic Attack, Transient (metabolism, pathology)
• Mice, Transgenic
• Time Factors
• Ubiquitin (metabolism)