Abstract |
Chinese three-striped box turtle (Cuora trifasciata), as a freshwater turtle, is used as a tonic food. The purpose of this study was to isolate peptides with cancer growth inhibition activity from trypsin-digested hydrolysates of turtle proteins. The results demonstrated that two fractions T1 and T2 exhibited good inhibition on HepG-2 and MCF-7 cancer cells, with an inhibition of 70.65-89.1%, at 500 μg/mL. Subsequently, three peptides were identified from T1 and T2, including RGVKGPR (T1-1), KLGPKGPR (T1-2), and SSPGPPVH (T2-1). By database search, T2-1 was a completely new peptide; its inhibition activity on MCF-7 cancer cells was the best, up to 70.02% at 500 μg/mL. Then, T1 and T2-1 were nanoencapsulated by chitosan. After nanoencapsulation, the inhibition percentages were 50.23% for the nanoencapsulated T1 on HepG-2 and 46.82% for the encapsulated T2-1 on MCF-7. The release experiment indicated that the encapsulated peptides could be slowly released in simulated gastrointestinal juice.
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Authors | Shengjie He, Xinliang Mao, Ting Zhang, Xiaolei Guo, Yazhong Ge, Chungwah Ma, Xuewu Zhang |
Journal | Journal of microencapsulation
(J Microencapsul)
Vol. 33
Issue 4
Pg. 344-54
(Jun 2016)
ISSN: 1464-5246 [Electronic] England |
PMID | 27292913
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Nanocapsules
- Peptides
- Chitosan
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Topics |
- Animals
- Antineoplastic Agents
(chemistry, isolation & purification, pharmacokinetics, pharmacology)
- Chitosan
(chemistry)
- Hep G2 Cells
- Humans
- MCF-7 Cells
- Nanocapsules
(chemistry)
- Peptides
(chemistry, isolation & purification, pharmacokinetics, pharmacology)
- Turtles
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